Anti-ZIP7 SLC39A7 Antibody

Product Name

Anti-ZIP7 SLC39A7 Antibody

View all SLC39A7/ZIP7 Antibodies

SKU/Catalog Number

A07719-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZIP7 SLC39A7 Antibody (Catalog # A07719-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ZIP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZIP7 SLC39A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07719-2)

Host

Rabbit

Contents

ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Anti-ZIP7 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Reactive Species

A07719-2 is reactive to SLC39A7 in Human, Mouse, Rat

Applications

A07719-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.118kDa

Background of SLC39A7/ZIP7

The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ZIP7, A07719-2 (1 μg/mL), ZIP7, (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Mouse Brain Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: ZIP7

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Western Blot Validation in Rat Brain Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: ZIP7

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Immunohistochemistry Validation of ZIP7 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (A07719-2) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Immunofluorescence Validation of ZIP7 in Human Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with A07719-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Gene/Protein Information For SLC39A7 (Source: Uniprot.org, NCBI)

Alternative Names

D6S115E; D6S2244EReally interesting new gene 5 protein; H2-KE4; HKE4Solute carrier family 39 member 7; HLA class II region expressed gene KE4; Ke4 gene, mouse, human homolog of; KE4; RING5Histidine-rich membrane protein Ke4; solute carrier family 39 (zinc transporter), member 7; zinc transporter SLC39A7; ZIP7 SLC39A7 D6S115E, D6S2244E, H2-KE4, HKE4, KE4, RING5, ZIP7 solute carrier family 39 member 7 zinc transporter SLC39A7|HLA class II region expressed gene KE4|Ke4 gene, mouse, human homolog of|histidine-rich membrane protein Ke4|really interesting new gene 5 protein|solute carrier family 39 (zinc transporter), member 7|zrt-, Irt-like protein 7

For more info on SLC39A7, check out the SLC39A7 Infographic

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In this infographic, you will see the following information for SLC39A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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