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Tnfrsf8 antibody (A01225-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEPA1-6 cells using anti-Tnfrsf8 antibody (A01225-1).
Overlay histogram showing HEPA1-6 cells stained with A01225-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf8 Antibody (A01225-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A01225-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf8 Antibody (A01225-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD30/Tnfrsf8 Picoband® Antibody
Tnfrsf8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Tnfrsf8 antibody was positively detected by WB in:
rat spleen tissue, rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse SP20 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Tnfrsf8 antibody was positively detected by FC in:
HEPA1-6 cell, NRK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CD30/Tnfrsf8 Picoband® Antibody
View all TNFRSF8 Antibodies
View all TNFRSF8 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD30/Tnfrsf8 recombinant protein (Position: D22-Q272).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
53.2 kDa
Background of Tnfrsf8
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. It is mapped to 4 E1; 4 78.17 cM. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Cite This Product
Anti-CD30/Tnfrsf8 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01225-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
82%
Moderate Homology
Human
55%
Low homology
Monkey
54%
Low homology
Rabbit
53%
Low homology
Cow
50%
Low homology
Sheep
47%
Low homology
Dog
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Tnfrsf8 antibody
Flow Cytometry (FC) protocol for Tnfrsf8 antibody
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Product Promise
We promise Anti-CD30/Tnfrsf8 Picoband® Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01225-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026