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Product Info Summary
| SKU: | A03300-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZIPK DAPK3 Antibody
SKU/Catalog Number
A03300-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIPK DAPK3 Antibody (Catalog # A03300-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZIPK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIPK DAPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03300-1)
Host
Rabbit
Contents
ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK.
Reactive Species
A03300-1 is reactive to DAPK3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.5 kDa
Background of DAPK3
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03300-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.
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Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
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Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
Specific Publications For Anti-ZIPK DAPK3 Antibody (A03300-1)
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