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Product Info Summary
| SKU: | A12174 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZKSCAN4/Znf307 Antibody
SKU/Catalog Number
A12174
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ZKSCAN4/Znf307 Antibody catalog # A12174. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZKSCAN4/Znf307 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12174)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
F7E4
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human ZKSCAN4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12174 is reactive to ZKSCAN4 in Human
Applications
A12174 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
61.579kDa
Background of ZNF307
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of MCF7, HepG2, 293, Mouse Brain, Mouse Lung cells using ZKSCAN4 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For ZKSCAN4 (Source: Uniprot.org, NCBI)
Gene Name
ZKSCAN4
Full Name
Zinc finger protein with KRAB and SCAN domains 4
Weight
61.579kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
EC 2.1.1.43; EC 4.2.1.52; FLJ32136; P1P373C6; p373c6.1; Zinc finger protein 307p373c6.1 (novel C2H2 type zinc finger protein); Zinc finger protein 427zinc finger protein with KRAB and SCAN domains 4; zinc finger with KRAB and SCAN domains 4; ZNF307P373c6.1; ZNF427 ZKSCAN4 P1P373C6, ZNF307, ZNF427, ZSCAN36 zinc finger with KRAB and SCAN domains 4 zinc finger protein with KRAB and SCAN domains 4|p373c6.1 (novel C2H2 type zinc finger protein)|zinc finger protein 307|zinc finger protein 427
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZKSCAN4, check out the ZKSCAN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZKSCAN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZKSCAN4/Znf307 Antibody (A12174)
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No publications found for A12174
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ZKSCAN4/Znf307 Antibody
Question
I see that the anti-ZKSCAN4/Znf307 antibody A12174 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-ZKSCAN4/Znf307 antibody A12174 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The anti-ZKSCAN4/Znf307 antibody (A12174) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-ZKSCAN4/Znf307 antibody A12174. Let me know if you need anything else.
R. Gonzalez
Verified customer
Asked: 2019-03-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-05
Question
Our lab want to know about to test anti-ZKSCAN4/Znf307 antibody A12174 on human embryonic kidney for research purposes, then I may be interested in using anti-ZKSCAN4/Znf307 antibody A12174 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
The products we sell, including anti-ZKSCAN4/Znf307 antibody A12174, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-25
Question
Does A12174 anti-ZKSCAN4/Znf307 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Huang
Verified customer
Asked: 2017-08-07
Answer
It shows on the product datasheet, A12174 anti-ZKSCAN4/Znf307 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-07
Question
Do you have a BSA free version of anti-ZKSCAN4/Znf307 antibody A12174 available?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZKSCAN4/Znf307 antibody A12174 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-22
Question
I was wanting to use your anti-ZKSCAN4/Znf307 antibody for WB for human embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryonic kidney identification?
A. Kulkarni
Verified customer
Asked: 2014-03-31
Answer
As indicated on the product datasheet, A12174 anti-ZKSCAN4/Znf307 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-31
