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Product Info Summary
| SKU: | A12954-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Zinc finger protein 420 ZNF420 Antibody
SKU/Catalog Number
A12954-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein 420 ZNF420 Antibody catalog # A12954-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zinc finger protein 420 ZNF420 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12954-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ZNF420. AA range:301-350
Reactive Species
A12954-1 is reactive to ZNF420 in Human, Mouse
Calculated molecular weight
80.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12954-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using ZNF420 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of ZNF420 Antibody. The lane on the right is blocked with the ZNF420 peptide.
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Western blot analysis of the lysates from COLO205 cells using ZNF420 antibody.
Specific Publications For Anti-Zinc finger protein 420 ZNF420 Antibody (A12954-1)
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Customer Q&As
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5 Customer Q&As for Anti-Zinc finger protein 420 ZNF420 Antibody
Question
We are currently using anti-ZNF420 antibody A12954-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-ZNF420 antibody (A12954-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
Is there a BSA free version of anti-ZNF420 antibody A12954-1 available?
B. Zhang
Verified customer
Asked: 2016-02-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZNF420 antibody A12954-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-25
Question
Does anti-ZNF420 antibody A12954-1 work for WB with placenta testis?
B. Baker
Verified customer
Asked: 2015-03-04
Answer
According to the expression profile of placenta testis, ZNF420 is highly expressed in placenta testis. So, it is likely that anti-ZNF420 antibody A12954-1 will work for WB with placenta testis.
Boster Scientific Support
Answered: 2015-03-04
Question
I see that the anti-ZNF420 antibody A12954-1 works with WB, what is the protocol used to produce the result images on the product page?
L. Johnson
Verified customer
Asked: 2014-11-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-11-28
Question
We are interested in to test anti-ZNF420 antibody A12954-1 on human placenta testis for research purposes, then I may be interested in using anti-ZNF420 antibody A12954-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Yang
Verified customer
Asked: 2014-08-27
Answer
The products we sell, including anti-ZNF420 antibody A12954-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-27
