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Product Info Summary
| SKU: | A08655 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZNF638/Np220 Antibody
SKU/Catalog Number
A08655
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZNF638/Np220 Antibody (Catalog # A08655). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNF638/Np220 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08655)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF638.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08655 is reactive to ZNF638 in Human
Applications
A08655 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
220.625kDa
Background of ZNF638
Early regulator of adipogenesis that works as a transcription cofactor of CEBPs, controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1 By similarity. Binds to cytidine clusters in double-stranded DNA.
Inagaki H., J. Biol. Chem. 271:12525-12531(1996).
Eichmueller S., Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001).
Hillier L.W., Nature 434:724-731(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, treated with serum (20%, 15mins), using ZNF638 antibody A08655.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ZNF638 antibody A08655.
Protein Target Info & Infographic
Gene/Protein Information For ZNF638 (Source: Uniprot.org, NCBI)
Gene Name
ZNF638
Full Name
Zinc finger protein 638
Weight
220.625kDa
Alternative Names
CTCL tumor antigen se33-1; CTCL-associated antigen se33-1; Cutaneous T-cell lymphoma-associated antigen se33-1; DKFZp686P1231; MGC26130; MGC90196; NP220 nuclear protein; NP220zinc finger, matrin-like; Nuclear protein 220; ZFML; Zfp638; Zinc finger matrin-like protein; zinc finger protein 638 ZNF638 NP220, ZFML, Zfp638 zinc finger protein 638 zinc finger protein 638|CTCL tumor antigen se33-1|CTCL-associated antigen se33-1|NP220 nuclear protein|cutaneous T-cell lymphoma-associated antigen se33-1|nuclear protein 220|zinc finger matrin-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF638, check out the ZNF638 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF638: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF638/Np220 Antibody (A08655)
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No publications found for A08655
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-ZNF638/Np220 Antibody
Question
Is there a BSA free version of anti-ZNF638/Np220 antibody A08655 available?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZNF638/Np220 antibody A08655 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-ZNF638/Np220 antibody A08655 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-ZNF638/Np220 antibody (A08655) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Does anti-ZNF638/Np220 antibody A08655 work on bovine IHC with epithelium?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not tested anti-ZNF638/Np220 antibody A08655 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ZNF638/Np220 antibody A08655 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine epithelium in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
Is this A08655 anti-ZNF638/Np220 antibody reactive to the isotypes of ZNF638?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
The immunogen of A08655 anti-ZNF638/Np220 antibody is Synthesized peptide derived from internal of human ZNF638. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-05
Question
I was wanting to use your anti-ZNF638/Np220 antibody for IHC for human cervix testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix testis identification?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
It shows on the product datasheet, A08655 anti-ZNF638/Np220 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-20
Question
My question regards to test anti-ZNF638/Np220 antibody A08655 on human cervix testis for research purposes, then I may be interested in using anti-ZNF638/Np220 antibody A08655 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Moore
Verified customer
Asked: 2016-03-25
Answer
The products we sell, including anti-ZNF638/Np220 antibody A08655, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-25
Question
Please see the WB image, lot number and protocol we used for cervix testis using anti-ZNF638/Np220 antibody A08655. Please let me know if you require anything else.
D. Li
Verified customer
Asked: 2013-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-22
