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Product Info Summary
| SKU: | A12285 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Zinc finger protein 7 ZNF7 Antibody
SKU/Catalog Number
A12285
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein 7 ZNF7 Antibody (Catalog# A12285). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zinc finger protein 7 ZNF7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12285)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ZNF7. Immunogen sequence location: 41-90
Reactive Species
A12285 is reactive to ZNF7 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
77.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12285 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using ZNF7 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ZNF7 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Zinc finger protein 7 ZNF7 Antibody (A12285)
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Customer Q&As
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4 Customer Q&As for Anti-Zinc finger protein 7 ZNF7 Antibody
Question
I was wanting to use your anti-ZNF7 antibody for IHC for human urinary bladder on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human urinary bladder identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
As indicated on the product datasheet, A12285 anti-ZNF7 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
Would anti-ZNF7 antibody A12285 work for IHC with urinary bladder?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to the expression profile of urinary bladder, ZNF7 is highly expressed in urinary bladder. So, it is likely that anti-ZNF7 antibody A12285 will work for IHC with urinary bladder.
Boster Scientific Support
Answered: 2020-03-16
Question
Can you help my question with product A12285, anti-ZNF7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12285 anti-ZNF7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-ZNF7 antibody A12285 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
C. Anderson
Verified customer
Asked: 2017-08-15
Answer
The anti-ZNF7 antibody (A12285) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-15
