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Product Info Summary
| SKU: | RP1106 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LFA3/CD58 Antibody Picoband®
SKU/Catalog Number
RP1106
PB1001 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LFA3/CD58 Antibody catalog # RP1106. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LFA3/CD58 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1106)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human LFA3 recombinant protein (Position: F29-R215).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1106 is reactive to CD58 in Human
Observed Molecular Weight
26 kDa
Calculated molecular weight
28.1 kDa
Background of CD58
CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1106 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106cells
Positive Control
WB: HELA whole cell (human tonsil tissue
FCM: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
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Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (RP1106).
Overlay histogram showing Hela cells stained with RP1106 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (RP1106,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-LFA3/CD58 Antibody Picoband® (RP1106)
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3 Customer Q&As for Anti-LFA3/CD58 Antibody Picoband®
Question
Would RP1106 anti-LFA3/CD58 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can see on the product datasheet, RP1106 anti-LFA3/CD58 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
I have attached the WB image, lot number and protocol we used for erythrocyte using anti-LFA3/CD58 antibody RP1106. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-LFA3/CD58 antibody RP1106 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
The anti-LFA3/CD58 antibody (RP1106) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-26
