Anti-ZSCAN4 Antibody

Product Name

Anti-ZSCAN4 Antibody

View all ZSCAN4 Antibodies

SKU/Catalog Number

A13391

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZSCAN4 Antibody (Catalog # A13391). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ZSCAN4 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZSCAN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13391)

Host

Rabbit

Contents

ZSCAN4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4.

Reactive Species

A13391 is reactive to ZSCAN4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.0 kDa

Background of ZSCAN4

Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13391 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ZSCAN4

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