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Product Info Summary
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Anti-ZWINT Antibody Picoband™
Boster Bio Anti-ZWINT Antibody Picoband™ catalog # A07023. Tested in ELISA, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZWINT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07023)
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
E.coli-derived rat ZWINT recombinant protein (Position: M1-D253).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A07023 is reactive to Zwint in Rat
A07023 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (A07023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # A07023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38KD. The expected band size for ZWINT is at 38KD.
Gene/Protein Information For Zwint (Source: Uniprot.Org, NCBI)
C20orf164; chromosome 20 open reading frame 164; dJ337O18.7; zinc finger SWIM domain-containing protein 3; zinc finger, SWIM domain containing 3; zinc finger, SWIM-type containing 3 Zwint|Sip30-1, Zwint|ZW10 interacting kinetochore protein|ZW10 interactor|SNAP25-interacting protein 30|ZW10 interacting protein-1|ZW10 interactor, kinetochore protein|neuroprotective protein 8|scoilin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Zwint, check out the Zwint Infographic
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In this infographic you will see the following information for Zwint: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A07023
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