ATHL1 (PGGHG) (NM_025092) Human Over-expression Lysate

Lysate of PGGHG over-expression cell lines, WB control

Transient overexpression lysate of ATH1, acid trehalase-like 1 (yeast) (ATHL1)

Product Info Summary

SKU: LS080162
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of ATH1, acid trehalase-like 1 (yeast) (ATHL1)

Product Name

ATHL1 (PGGHG) (NM_025092) Human Over-expression Lysate

See all PGGHG products

SKU/Catalog Number

LS080162

Size

100 µg

Description

Transient overexpression lysate of ATH1, acid trehalase-like 1 (yeast) (ATHL1)

Storage & Handling

Cite This Product

ATHL1 (PGGHG) (NM_025092) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080162)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PGGHG (Source: Uniprot.org, NCBI)

Gene Name

PGGHG

Full Name

Protein-glucosylgalactosylhydroxylysine glucosidase

Weight

80.655kDa

Superfamily

glycosyl hydrolase 65 family

Alternative Names

Protein-glucosylgalactosylhydroxylysine glucosidase PGGHG ATHL1 protein-glucosylgalactosylhydroxylysine glucosidase protein-glucosylgalactosylhydroxylysine glucosidase|ATH1, acid trehalase-like 1|acid trehalase-like protein 1|testicular tissue protein Li 25

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PGGHG, check out the PGGHG Infographic

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