Product Info Summary
SKU: | LS000515 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (ATP5F1), nuclear gene encoding mitochondrial protein |
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Product info
Product Name
ATP5PB (NM_001688) Human Over-expression Lysate
SKU/Catalog Number
LS000515
Size
100 µg
Description
Transient overexpression lysate of ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (ATP5F1), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
ATP5PB (NM_001688) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000515)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ATP5PB Human Over-expression Lysates NM_001688.
Western blot validation of overexpression lysate (LS000515) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ATP5F1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ATP5PB (Source: Uniprot.org, NCBI)
Gene Name
ATP5PB
Full Name
ATP synthase F(0) complex subunit B1, mitochondrial
Weight
28.909kDa
Superfamily
eukaryotic ATPase B chain family
Alternative Names
ATP synthase B chain, mitochondrial; ATP synthase subunit b, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATPase subunit b; cell proliferation-inducing protein 47; H+-ATP synthase subunit b; MGC24431; PIG47 ATP5PB ATP5F1, PIG47 ATP synthase peripheral stalk-membrane subunit b ATP synthase F(0) complex subunit B1, mitochondrial|ATP synthase B chain, mitochondrial|ATP synthase proton-transporting mitochondrial F(0) complex subunit B1|ATP synthase subunit b, mitochondrial|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b|ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1|ATPase subunit b|H+-ATP synthase subunit b|cell proliferation-inducing protein 47
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ATP5PB, check out the ATP5PB Infographic
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In this infographic, you will see the following information for ATP5PB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ATP5PB (NM_001688) Human Over-expression Lysate (LS000515)
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