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Product Info Summary
| SKU: | LS000527 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c (ATP6V0C) |
Product info
Product Name
ATP6V0C (NM_001694) Human Over-expression Lysate
SKU/Catalog Number
LS000527
Size
100 µg
Description
Transient overexpression lysate of ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c (ATP6V0C)
Storage & Handling
Cite This Product
ATP6V0C (NM_001694) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000527)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ATP6V0C Human Over-expression Lysates NM_001694.
Western blot validation of overexpression lysate (LS000527) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ATP6V0C (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ATP6V0C (Source: Uniprot.org, NCBI)
Gene Name
ATP6V0C
Full Name
V-type proton ATPase 16 kDa proteolipid subunit
Weight
15.736kDa
Superfamily
V-ATPase proteolipid subunit family
Alternative Names
ATP6CATPase, H+ transporting, lysosomal (vacuolar proton pump) 16kD; ATP6LVPPC; ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c; ATPL; H(+)-transporting two-sector ATPase, 16 kDa subunit; vacuolar ATP synthase 16 kDa proteolipid subunit; vacuolar H+ ATPase proton channel subunit; Vacuolar proton pump 16 kDa proteolipid subunit; VATL; V-ATPase 16 kDa proteolipid subunit; Vma3; V-type proton ATPase 16 kDa proteolipid subunit ATP6V0C ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3 ATPase H+ transporting V0 subunit c V-type proton ATPase 16 kDa proteolipid subunit|ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c|H(+)-transporting two-sector ATPase, 16 kDa subunit|V-ATPase 16 kDa proteolipid subunit|vacuolar ATP synthase 16 kDa proteolipid subunit|vacuolar H+ ATPase proton channel subunit|vacuolar proton pump 16 kDa proteolipid subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ATP6V0C, check out the ATP6V0C Infographic
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In this infographic, you will see the following information for ATP6V0C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ATP6V0C (NM_001694) Human Over-expression Lysate (LS000527)
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