Apoptosis, or programmed cell death, is a fundamental process in maintaining cellular homeostasis and plays a pivotal role in various physiological and pathological conditions. Accurate detection of apoptosis is essential for understanding disease mechanisms, evaluating drug efficacy, and advancing biomedical research. Among the myriad of techniques available, Annexin V/Propidium Iodide (PI) staining coupled with flow cytometry stands out as a powerful and reliable method for detecting and quantifying apoptotic cells. This comprehensive guide aims to equip scientists and researchers with the knowledge and practical steps needed to optimize apoptosis detection using Annexin V/PI staining in flow cytometry.
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