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Product Info Summary
| SKU: | LS140690 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of CCCTC-binding factor (zinc finger protein)-like (CTCFL) |
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Product info
Product Name
BORIS (CTCFL) (NM_080618) Human Over-expression Lysate
SKU/Catalog Number
LS140690
Size
100 µg
Description
Transient overexpression lysate of CCCTC-binding factor (zinc finger protein)-like (CTCFL)
Storage & Handling
Cite This Product
BORIS (CTCFL) (NM_080618) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS140690)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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BORIS (CTCFL) Human Over-expression Lysates NM_080618.
Western blot validation of overexpression lysate (LS140690) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CTCFL (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CTCFL (Source: Uniprot.org, NCBI)
Gene Name
CTCFL
Full Name
Transcriptional repressor CTCFL
Weight
75.747kDa
Superfamily
CTCF zinc-finger protein family
Alternative Names
BORISHMGB1L1; BORIS-like protein; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC-binding factor (zinc finger protein)-like; CCCTC-binding factor; CT27MGC163358; CTCF paralog; CTCF-like protein; CTCF-T; dJ579F20.2; HMG-1L1; MGC169105; MGC169106; putative high mobility group protein 1-like 1; putative high mobility group protein B1-like 1; transcriptional repressor CTCFL; Zinc finger protein CTCF-T CTCFL BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2 CCCTC-binding factor like transcriptional repressor CTCFL|BORIS-like protein|brother of the regulator of imprinted sites|cancer/testis antigen 27
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CTCFL, check out the CTCFL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTCFL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BORIS (CTCFL) (NM_080618) Human Over-expression Lysate (LS140690)
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