AQP9 · Western blot design guide

AQP9 Western Blot Planning Guide

Plan an AQP9 Western blot around the catalog-observed 37.2 kDa band, image-backed PA2094 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for AQP9 (AQP9): expected band 37.2 kDa, antibody PA2094, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

AQP9 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	37.2 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Liver
	Negative control ⓘ	Bone marrow

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	37.2 kDa
ⓘ	Localization	Cell membrane / Cytoplasmic vesicle membrane
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Mouse

Sample controls for AQP9 Western blot

🧪Use Liver as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.

Positive control: Liver (Medium)

Negative control: Bone marrow (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for AQP9

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Liver	Reported tissue cells	Medium	Protein (HPA)	HPA →
Appendix	Reported tissue cells	Low	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Bone marrow	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Bronchus	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected AQP9 Western Blot Band Size?

Use the product-observed 37.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
37.2 kDa	Matches the authoritative product WB observation.
37.2 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected AQP9 appearancePlan around 37.2 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	37.2 kDa; use this as the primary experimental expectation.
Calculated mass	37.2 kDa from UniProt O14520; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with PA2094.

Why is my band missing or off?
Situation	Likely cause	Next action
37.2 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt O14520 Human Protein Atlas product datasheet (PA2094)

Experiment evidence

Western blot images showing AQP9 band

PA2094 · AQP9 — observed 37.2 kDa WB validation

PA2094 · AQP9Observed 37.2 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated AQP9 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Liver
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	PA2094 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced AQP9 Western Blot Tips

Deeper troubleshooting and optimisation questions for AQP9, answered from its protein features.

Which band should guide the blot?

Use 37.2 kDa, the observation attached to the authoritative PA2094 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 37.2 kDa expectation.

Which positive control should I start with?

Start with Liver, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Bone marrow as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for AQP9 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should PA2094 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC2944173, PMC8678610.

Boster reagents

AQP9 Western Blot Reagents

Mouse-reactive AQP9 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for AQP9 using PA2094; observed band 37.2 kDa

Anti-Aquaporin 9/AQP9 Antibody Picoband®

Cat # PA2094

Reactivity: Mouse

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.