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- Table of Contents
ATP11C · Western blot design guide
ATP11C Western Blot Planning Guide
Plan an ATP11C Western blot around the catalog-observed 129.5 kDa band, image-backed A06516-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ATP11C Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 129.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Colon | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 129.5 kDa |
| ⓘ | Localization | Cell membrane / Endoplasmic reticulum membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated ATP11C Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A06516-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ATP11C Western Blot Band Size?
Use the product-observed 129.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 129.5 kDa | Matches the authoritative product WB observation. |
| 129.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ATP11C appearancePlan around 129.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 129.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 129.5 kDa from UniProt Q8NB49; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A06516-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 129.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ATP11C band
A06516-1 · ATP11C
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ATP11C Western blot
🧪Use Colon as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Colon (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ATP11C
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Small intestine | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced ATP11C Western Blot Tips
Deeper troubleshooting and optimisation questions for ATP11C, answered from its protein features.
Which band should guide the blot?
Use 129.5 kDa, the observation attached to the authoritative A06516-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 129.5 kDa expectation.
Which positive control should I start with?
Start with Colon, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ATP11C Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A06516-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ATP11C Western Blot Reagents
Human-reactive ATP11C Western blot reagents with authoritative product imagery.
Real WB data
Anti-ATP11C Antibody Picoband®
Cat # A06516-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q8NB49
- Human Protein Atlas — ATP11C
- A06516-1 product record
- PMC7754070 — Reduction in flippase activity contributes to surface presentation of phosphatidylserine in human senescent erythrocytes (Journal of cellular and molecular medicine, 2020)
- PMC13382312 — Clinical variability of ATP11C-related hemolytic anemia: expanding the phenotypic and diagnostic spectrum (Blood advances, 2026)
- PMC5680300 — Phospholipid flippase ATP11C is endocytosed and downregulated following Ca(2+)-mediated protein kinase C activation (Nature communications, 2017)
