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Alkbh8 Antibodies
AND Alkbh8 ELISA kits, Alkbh8 Proteins
Many biological assays use antibodies to detect Alkbh8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Alkbh8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Alkbh8 antibodies...
Designing an IHC experiment for Alkbh8? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ALKBH8 IHC GuideAlkbh8 Infographic by Boster Bio
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Alkbh8 Overview
Facts about Alkylated DNA repair protein alkB homolog 8.
Alkylated DNA repair protein alkB homolog 8 (Alkbh8)
Catalyzes the methylation of 5-carboxymethyl uridine into 5-methylcarboxymethyl uridine in the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966). Catalyzes the previous step in the formation of 5- methylcarboxymethyl uridine in the wobble position of the anticodon loop in target tRNA (PubMed:20123966).
Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys) (By similarity). Binds tRNA and catalyzes the iron and alpha- ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine in the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)- methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:20583019).
Gene Information
| Mouse | |
|---|---|
| Gene Name: | Alkbh8 |
| Uniprot: | Q80Y20 |
| Entrez: | 67667 |
Family
| Belongs to: |
|---|
| alkB family |
Alternative Names
ABH8MGC10235; AlkB homologue 8; alkB, alkylation repair homolog 8 (E. coli); alkylated DNA repair protein alkB homolog 8; EC 1.14.11.-; EC 2.1.1.-; FLJ38204; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
Molecular Weight
Mass (kDA):
74.768 kDA
Genomic Context
| Mouse | |
|---|---|
| Location: | 9|9 A1 |
| Sequence: | 9; |
Diseases
- Carcinoma, Transitional Cell
- Bladder Neoplasm
- Cell Invasion
- Carcinoma In Situ
- Tumor Angiogenesis
- Malignant Neoplasms
- Pathologic Neovascularization
Pathways
- Methylation
- Dna Repair
- Translation
- Rna Modification
- Trna Modification
- Cell Death
- Trna Methylation
PTMs
- Hydroxylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Alkbh8
bosterbio.com/bosterbio-gene-info-cards/Alkbh8
References
PMID: 20583019 by Fu Y., et al. The AlkB domain of mammalian ABH8 catalyzes hydroxylation of 5- methoxycarbonylmethyluridine at the wobble position of tRNA.
PMID: 20123966 by Songe-Moller L., et al. Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.