BCAR1 Antibodies

AND BCAR1 ELISA kits, BCAR1 Proteins

Many biological assays use antibodies to detect BCAR1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BCAR1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BCAR1 antibodies...

We validate the specificity of these antibodies to BCAR1 by testing them on tissues known to express BCAR1 positively and negatively. Browse below to find the BCAR1 antibody that suites your experiment. We have 10 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for BCAR1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the BCAR1 Western Blot Guide

BCAR1 Infographic by Boster Bio

All BCAR1 Products

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Product

Figures

Specificity

Applications

Price

Anti-Phospho-BCAR1 (Y410) Rabbit Monoclonal Antibody

Western blot analysis of Phospho-BCAR1 (Y410) expression in HeLa treated with pervanadate cell lysate

Human

$370
Cat # M00960Y410
100 µl/vial

Anti-BCAR1 Rabbit Monoclonal Antibody

Western blot analysis of BCAR1 expression in A431 cell lysate

Human, Mouse, Rat

$370
Cat # M00960-2
100 μl/vial

BCAR1 (Phospho-Tyr249) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30

Human, Mouse, Rat

ELISA

$540
Cat # EKC2168
1 kit, containing two 96-well plates and all necessary reagents

BCAR1 (Phospho-Tyr165) Colorimetric Cell-Based ELISA Kit

Human, Mouse, Rat

ELISA

$540
Cat # EKC2167
1 kit, containing two 96-well plates and all necessary reagents

BCAR1 (Phospho-Tyr410) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from NIH-3T3 cells

Human, Mouse, Rat

ELISA

$540
Cat # EKC1991
1 kit, containing two 96-well plates and all necessary reagents

BCAR1 Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HuvEc cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1054
1 kit, containing one 96-well plate and all necessary reagents

BCAR1 (NM_014567) Human Over-expression Lysate

Human

$628
Cat # LS009564
20 µg, 100 µg

Human p130Cas Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

SDS-PAGE, Denatured

$265
Cat # PROTP56945
Starting from 10ug

ETK (Phospho-Tyr566) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with Serum 20% 15

Human, Mouse

ELISA

$540
Cat # EKC2195
1 kit, containing two 96-well plates and all necessary reagents

ETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HepG2 cells

Human, Mouse

ELISA

$540
Cat # EKC2153
1 kit, containing two 96-well plates and all necessary reagents

BCAR1 Overview

Facts about Breast cancer anti-estrogen resistance protein 1.

BCAR1 3D structure. Source: alphafold

Breast cancer anti-estrogen resistance protein 1 (BCAR1)

Docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration.

Overexpression confers antiestrogen resistance on breast cancer cells. .

Gene Information

Human
Gene Name:	BCAR1
Uniprot:	P56945
Entrez:	9564

Family

Belongs to:

CAS family

Alternative Names

BCAR1; breast cancer estrogen resistance 1; Cas scaffolding protein family member 1; CAS1; CASFLJ45059; CASS1; CASS1CRKASbreast cancer estrogen resistance protein 1; CRKAS; Crk-associated substrate p130Cas; CRK-associated substrate; FLJ12176; p130Cas

Molecular Weight

Mass (kDA):
93.372 kDA

Genomic Context


Human

Location:	16q23.1
Sequence:	16; NC_000016.10 (75228181..75268007, complement)

Tissue Specificity

Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Subcellular Localizations

Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

Diseases

Tissue Adhesions
Malignant Neoplasms
Neoplasms
Malignant Neoplasm Of Breast
Mammary Neoplasms
Neoplasm Metastasis
Cell Transformation, Neoplastic
Cell Invasion
Carcinoma
Neoplasm Invasiveness
Malignant Paraganglionic Neoplasm
Metaplastic Cell Transformation
Retinoblastoma

Melanoma
Tumor Progression
Prostatic Neoplasms
Malignant Neoplasm Of Prostate
Malignant Neoplasm Of Lung
Carcinogenesis
Leukemia

Interacting Proteins

CRK
FYN
INPPL1
IQGAP1
MMP14

PEAK1
PTK2
PTPN1
PTPN12
SRC

SRCIN1
Srcin1
TNK2
TNS3

Pathways

Cell Migration
Cell Adhesion
Localization
Cell Proliferation
Cell Cycle
Cell Growth
Cell Motility
Cell Death
Secretion
Hyperphosphorylation
Rna Interference
Pathogenesis
Chemotaxis

Phagocytosis
Anoikis
Cytoskeleton Organization
Proteolysis
Actin Cytoskeleton Organization
Mitosis
Cell Differentiation

PTMs

Phosphorylation
Dephosphorylation
Cleavage
Ribosylation
Acetylation
Nitration

Nitrosylation
Amidation
Oxidation
Methylation
Prenylation
Palmitoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCAR1

References

PMID: 10639512 by Brinkman A., et al. BCAR1, a human homologue of the adapter protein p130Cas, induces anti-estrogen resistance in breast cancer cells.

PMID: 10587647 by Eisenmann K.M., et al. Melanoma chondroitin sulphate proteoglycan regulates cell spreading through Cdc42, Ack-1 and p130cas.