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- Table of Contents
1 Citations 8 Q&As
Facts about Biglycan.
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Human | |
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Gene Name: | BGN |
Uniprot: | P21810 |
Entrez: | 633 |
Belongs to: |
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small leucine-rich proteoglycan (SLRP) family |
BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A
Mass (kDA):
41.654 kDA
Human | |
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Location: | Xq28 |
Sequence: | X; NC_000023.11 (153494980..153509546) |
Found in several connective tissues, especially in articular cartilages.
Secreted, extracellular space, extracellular matrix.
Boster's primary antibody offers many advantages. Boster's primary antibodies have high-affinity and have been used and validated in research over the past 25 years. In addition, these antibodies have been validated for use in ELISA, Immunohistochemistry, and Western Blotting. Read on to find out more about Boster's primary antibodies. Learn how you can use the BGN marker to aid in your research.
Flow cytometry can be utilized in many areas of medicine and biology. Flow cytometry employs particles or cells as samples for detecting many biological processes. Boster Bio offers high-affinity primary antibodies, both monoclonal and polyclonal. These antibodies have a stellar record of scientific citations and have been in use for over 25 years.
Boster Bio offers more than 16,000 antibodies of high quality and validated for IHC, WB, ELISA, FC, and ELISA applications. You can also get free secondary antibodies when you purchase any of their primary antibodies. Boster Bio high-affinity primary antibodies are also available for mouse, human, and rat samples. Boster Bio's antibodies provide high levels of biological activity and sensitivity and are available in various sizes including milligrams and nanoliters.
This method of the elution process, antibodies were placed on sections and then crosslinked with species-specific FITC-conjugated secondary antibodies. They were stained either with or without prior elution depending on whether the initial or FITC staining of hapten was negative. Monomeric fragments bonded to the primary antibody layer stop staining by the secondary antibody.
Screening individual B cells for antibodies with high affinity against an antibody of interest takes only two weeks. The viable cells are usually isolated from convalescent people or immunized animals. Following that, the single-cell sorting procedure extracts VH/VL antibody genes that are paired to be evaluated and expressed. The high-affinity antibody isolated from the samples are extremely concentrated and suitable for clinical and research applications.
To determine whether Boster Bio high-affinity primary antibodies are appropriate for your specific needs first, determine the antigen-antibody sequences. 13I1, the most efficient antibody was identified as having 99.2 percent sequence similarity, which indicates a high affinity for target antigens. Further testing for application is conducted on the chosen antibodies. The most effective antibodies are used in clinical trials. These antibodies are also available in commercial quantities.
The antibodies isolated showed high affinity to the SARS-CoV-2 receptor binding domain. To determine their affinity, the antibodies were tested through flow cytometry for three separate experiments. The binding affinity of high-affinity antibodies (12H2 & 13I1) was significantly greater than that of the positive control antibody CB6. The results are presented as half-maximal effective concentrations (IC50) values based on at least three independent tests.
The heavy chains of the purified antibody were dispersed in 20 mM Acetate (pH 5.0) to determine their purity. The samples were then exchanged to PBS (pH 7.4), and then centrifuged at 3500xg over 40 minutes. Boster Bio recommends the use of the protein A beads to determine the purity of antibodies. Similar to that, Boster Bio antibodies can be useful for confirming the purity of protein-bound proteins.
Picokine(tm), Quick ELISA Kits make it simple to measure PICOKINE in as little as 90 minutes. Additionally, you can get outstanding results. The PicoKine(r) Quick ELISA kits provide high sensitivity, a simplified protocol, and superior repeatability. The kits are simple to use and can be used for testing a wide range of biological samples.
All the reagents needed conduct the test are contained in ELISA kits. ELISA is a plate-based method where antibodies are linked to enzymes. The results are quantified using the aid of enzymes. There are many formats available, and the principle of the interaction between antibody and antigen is different for each one. Picokine(tm), ELISA kits are high-affinity antibody that detect native proteins. Boster's QC department also validates the ELISA kit against immunogenic proteins and superfamilies.
Boster Bio uses human fetuin A PicoKine(tm), ELISA kits to determine levels in supernatants of cells. The Human Fetuin-A PicoKine(tm), ELISA kit included the anti-human Fetuin-A antibody. It was then added to 130mL supernatant of cells. Then, the Avidin-Biotin-Peroxidase Complex was added to the wells, and the reaction was performed at 37 degC.
Boster Bio PICOKINE (t) ELISA kits include matching antibody pairs which are designed to be optimized for ELISA development. The kits are available in various sizes and include all the reagents needed to run the test. The ELISA kits are also available in convenient packaging options that include an all-in one buffer kit.
Picokine(tm), ELISA kits, are a family high-sensitivity ELISAs, which use the exclusive coating and blocking technology. These ELISA kits feature a picogram level of sensitivity and have very low background. They can be tested against a range of biological matrices and cell culture supernatants and other substances to ensure reproducibility and lot consistency. Visit the Boster website for more information.
The Human High Sensitivity C-Reactive Protein ELISA Kit can test serum or plasma. This test is extremely sensitive, with a sensitivity level of 10 per milliliter. It is available in kits with 96 wells and disposable strips. The Human PSP94 ELISA Kit is sensitive to 10 pg/ml and is not cross-reactive with other relevant proteins.
Many species can be tested with commercial ELISA kits. If you are testing a species that is unique pick a kit that is validated for homology to the species of interest. This means that at least 90 percent of the antibody are compatible with one another. The specificity of these ELISA kits is even greater when they are validated for use on cells, tissues, and serum. They are also guaranteed to be consistent between batches and tests.
The process of conducting an ELISA requires careful sample preparation and the right choice of antibody concentration. Many sources of error can easily be eliminated by having the proper controls. Troubleshooting guides as well as technical blogs and other resources can assist in resolving any problems you may have. The technical blog also has articles on various aspects of ELISA application. These articles provide a detailed explanation of how ELISAs work, as well suggesting specific products for specific applications.
Boster ELISA kits are designed to detect more than 85 unique analytes within mouse, human, and rat tissues. Boster ELISA kits have been mentioned in more than 20000 scientific papers and are backed by over 25 years of experience. You can pick from a broad selection of Boster bio high-sensitivity ELISA kits to conduct several experiments. Because they have been tested against a variety of proteins, they offer the highest sensitivity.
Boster Bio offers several high-sensitivity ELISA products that support Neuroscience research. The company has been the world's most renowned OEM supplier of neurotrophins for over 30 years. The cytokine product line includes the reagents needed for metabolism and neurodevelopment and stress disorders. These products are manufactured using highly concentrated antigens and come with 16 distinct capture antibodies to enhance sensitivity.
PMID: 2647739 by Fisher L.W., et al. Deduced protein sequence of bone small proteoglycan I (biglycan) shows homology with proteoglycan II (decorin) and several nonconnective tissue proteins in a variety of species.
PMID: 1860845 by Fisher L.W., et al. Human biglycan gene. Putative promoter, intron-exon junctions, and chromosomal localization.
*More publications can be found for each product on its corresponding product page