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- Table of Contents
C1QBP · Western blot design guide
C1QBP Western Blot Planning Guide
Plan a C1QBP Western blot around the catalog-observed 31.4 kDa band, image-backed PB9921 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
C1QBP Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 31.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Colon | |
| Negative control | Skeletal muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 31.4 kDa |
| ⓘ | Localization | Mitochondrion matrix / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated C1QBP Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9921 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected C1QBP Western Blot Band Size?
Use the product-observed 31.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 31.4 kDa | Matches the authoritative product WB observation. |
| 31.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected C1QBP appearancePlan around 31.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 31.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 31.4 kDa from UniProt Q07021; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9921. |
| Situation | Likely cause | Next action |
|---|---|---|
| 31.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing C1QBP band
PB9921 · C1QBP
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for C1QBP Western blot
🧪Use Colon as the first positive-control candidate and Skeletal muscle as the HPA Not detected negative candidate.
Positive control: Colon (High)
Negative control: Skeletal muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for C1QBP
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Ovary | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced C1QBP Western Blot Tips
Deeper troubleshooting and optimisation questions for C1QBP, answered from its protein features.
Which band should guide the blot?
Use 31.4 kDa, the observation attached to the authoritative PB9921 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 31.4 kDa expectation.
Which positive control should I start with?
Start with Colon, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Skeletal muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for C1QBP Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9921 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
C1QBP Western Blot Reagents
Human/Mouse/Rat-reactive C1QBP Western blot reagents with authoritative product imagery.
Real WB data
Anti-GC1q R/C1QBP Antibody Picoband®
Cat # PB9921
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q07021
- Human Protein Atlas — C1QBP
- PB9921 product record
- PMC9800418 — Hyaluronic acid ameliorates intervertebral disc degeneration via promoting mitophagy activation (Frontiers in bioengineering and biotechnology, 2022)
- PMC3512559 — Complement 1q-binding protein inhibits the mitochondrial permeability transition pore and protects against oxidative stress-induced death (The Biochemical journal, 2011)
- PMC11534557 — Endogenous complement 1q binding protein (C1qbp) regulates mitochondrial permeability transition and post-myocardial infarction remodeling and dysfunction (Journal of molecular and cellular cardiology, 2024)
