Calnexin Antibodies

Calnexin (CANX)

Calcium-binding protein which interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It could behave in helping protein assembly or at the retention within the ER of unassembled protein subunits.

It seems to play a significant role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes which escape the ER of immature thymocytes, it may be a signaling complex regulating thymocyte maturation.

Gene Information

Human
Gene Name:	CANX
Uniprot:	P27824
Entrez:	821

Family

Belongs to:
calreticulin family

Alternative Names

calnexin; CNX; IP90FLJ26570; Major histocompatibility complex class I antigen-binding protein p88; P90

Molecular Weight

Mass (kDA):
67.568 kDA

Genomic Context

Human
Location:	5q35.3
Sequence:	5; NC_000005.10 (179678646..179731641)

Subcellular Localizations

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).

Diseases

• Endoplasmic Reticulum Stress
• Carcinoma
• Malignant Neoplasms
• Neoplasms
• Retinal Degeneration
• Hepatitis B
• Hypoxia
• Lung Diseases
• Infertility
• Malignant Neoplasm Of Lung
• Edema
• Sarcoidosis

• Adenocarcinoma
• Rectal Carcinoma
• Milroy Disease
• Male Infertility
• Liver Carcinoma
• Nerve Degeneration

Interacting Proteins

• ABCA1
• CFTR
• CHST15
• NDRG1
• OPRD1

• RPL12
• SEC61A1
• SSR1

Pathways

• Fertilization
• Regeneration
• Reverse Transcription
• Cell Proliferation
• Translation
• Cell Death
• Sperm Capacitation
• Mating

• Wound Healing
• Rna Processing
• Autophagy

PTMs

• Acetylation
• Disulfide bond
• Lipoprotein

• Palmitate
• Phosphoprotein
• Ubl conjugation

Research Areas

• Unfolded Protein Response
• Vision

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CANX

Best Uses Of The CANX Marker, Primary Antibodies, And Immunohistochemistry

In this article, we'll cover the best uses of the CANX Marker, primary antibodies, and Immunohistochemistry. You'll also learn about ELISA kits and other products for biomedical research. These products can help you with your research projects, from the earliest stages of a project to the most complex ones. Using these tools properly is essential for your project's success.

CANX Marker

The CANX marker is a protein produced by tumor cells that can be used to identify different types of cancer. When measured in a person's blood, tumor markers can indicate whether a cancer has spread or is aggressive. They can also provide insight into the response to a particular treatment. As the first tool for detecting prostate cancer, the CANX marker could soon be used for screening. However, the clinical context must be considered before using this test.

Immunohistochemistry

Antibodies against Calnexin are used in many biological assays. These antibodies can be polyclonal or monoclonal and react with Calnexin in many animal samples. Boster Bio develops their Calnexin antibodies using mouse and rabbit as the models for the assays. Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum (ER). In addition, it may play a role in the assembly of proteins by retaining unassembled subunits in the ER.

ELISA kits

CANX is a common cancer biomarker. The Boster Bio ELISA kits using this marker measure the presence of CANX in samples of human plasma. The assay uses specific antibodies to bind the target antigen and a detection system to detect the binding. To improve sensitivity, ELISA kits should be coated with high-affinity antibodies. Boster Bio has mastered the process of high-affinity antibody coating.

The Boster Bio ELISA kit is highly sensitive and allows you to determine the concentration of the CANX protein in a sample of human plasma. It detects levels as low as 0.3 ng/ml, which is suitable for immunoassays in many applications. The kit is available for both human and rat plasma. CANX is a protein that is highly expressed in the body and is responsible for many diseases and conditions.

Boster Biologicals develops ELISA kits and research antibodies to detect a variety of biomarkers. They can detect CANX in a wide variety of samples. The company has nearly 20 years of experience manufacturing high-quality ELISA kits and immunological reagents. The company has earned the respect of customers in the immunology community for its high-affinity antibodies. The company also consistently improves their techniques and protocols in order to produce antibodies with higher affinity.

For a high-sensitivity CANX ELISA, it's critical to select a kit that uses a non-colorimetric substrate. The CANX marker is not widely used, but it is useful for studies of cancer cells in human tissues and blood. In addition, the CANX ELISA kits can be adapted to measure the concentration of human CANX protein.

Primary antibodies

The CANX marker allows you to identify a range of specific antibodies against a range of antigens. Its unique complementarity-determining region is particularly useful for identifying primary antibodies. To learn more about complementarity-determining regions, visit the Wikipedia article. Primary antibodies are made by immunizing host animals with antigens. The antibodies are then purified from the host eggs or sera.

CANX is a common antigen, which is a good target for a number of immunohistochemical methods. This method uses antibody-antigen interactions to detect specific proteins in a range of tissues. Scientists optimize sample preparation and staining processes to obtain a strong signal. The results can then be interpreted accordingly. The CANX marker has been validated with multiple methods to confirm its effectiveness.

Boster Bio: The CANX marker can help scientists find a variety of primary antibodies for many applications. It is especially useful for detecting a wide range of protein molecules, such as cytokinesis-related proteins. It is used in biotech companies, including pharmaceutical and biotech companies. Agrisera: Agrisera is a Swedish company that manufactures monoclonal and polyclonal antibodies for plant protein analysis. Its products have been cited in thousands of scientific publications and were recently awarded Plant Science Antibody Supplier of the Year.

A variety of other companies supply monoclonal and polyclonal antibodies for diagnostic and medical applications. The company also produces a large number of antibodies. Its primary antibodies are targeted against druggable proteins, such as receptors, transporters, and neurobiologicals. The website offers customer reviews and protocols to help identify specific antibodies for various applications. The website has more than 40,000 monoclonal and polyclonal antibodies for various applications.

Secondary antibodies

Secondary antibodies using the CANX marker are widely used as a detection method for cancer. These antibodies can be used across multiple applications and provide the capability of multi-labeling and multiplexing. These antibodies are produced by immunizing the host animal with an antibody from a different species. Goat, rabbit, and donkey are commonly used as hosts. A primary antibody is generated in a rabbit cell culture.

Single-step labeling and secondary antibody saturation were determined using magnetophoretic mobility measurements. The primary objective was to determine the maximum magnetophoretic mobility of the secondary antibody. The antibodies were used in larger amounts than manufacturers recommend for a single-step labeling procedure. The concentration of the secondary magnetic colloid-conjugated antibody is a critical step in antibody labeling, especially when detecting rare cells.

Using an antibody to detect cancer cells is a common and useful method. It is useful for diagnosing many types of cancer, from advanced stomach cancer to breast cancer. Other common cancer markers are HER2, human chorionic gonadotropin, and prostate specific antigen. In cancer research, antibodies to these proteins are widely used to detect tumor markers. Some of them can be used in immunoassays, as well.

Combinatorial screening allows for the profiling of large numbers of bispecific antibodies in a single experiment. A droplet-based microfluidic platform allows the rapid generation of millions of antibodies. This platform maintains the genotype-phenotype linkage and enables the product from the previous round of selection to be amplified in the next round. It is also possible to enrich rare hits with several rounds of iteration, which reduces the cost of a single round of selection.