Rabbit Polyclonal antibody for CANX detection. Tested positive for IF, IF, IHC, IP, WB in Human, Mouse, Rat
|Reactivity||Human, Mouse, Rat|
|Applications||IP, IF, IHC, WB|
|Product Name||Anti-Calnexin Antibody
See all CANX primary antibodies, ELISA kits and proteins
|Storage & Handling||-20°C|
|Description||Rabbit Polyclonal antibody for CANX detection. Tested positive for IF, IF, IHC, IP, WB in Human, Mouse, Rat|
|Cite This Product||Anti-Calnexin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03372)|
|Specificity||Anti-Calnexin Antibody (A03372) reacts with Human, Mouse, Rat CANX, in native form and recombinant. Superfamily members of CANX are not reactive to A03372.|
|Contents/Buffer||Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.|
|Form||Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.|
|Immunogen||Synthetic peptide corresponding to the sequence near the C-terminus of dog calnexin. The sequence is completely conserved in human, mouse and rat.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Calnexin Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Images And Assay Conditions
Figure 1. IHC analysis of CANX using anti-CANX antibody (A03372).
CANX was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CANX Antibody (A03372) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. Western blot analysis of CANX using anti-CANX antibody (A03372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CANX antigen affinity purified polyclonal antibody (Catalog # A03372) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CANX.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
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