Anti-Calnexin Antibody

Rabbit Polyclonal antibody for CANX detection. Tested positive for IF, IF, IHC, IP, WB in Human, Mouse, Rat

SKU A03372
Size 200µg
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications IP, IF, IHC, WB

Overview

Product Name Anti-Calnexin Antibody
See all CANX primary antibodies, ELISA kits and proteins
SKU/Catalog Number A03372
Storage & Handling -20°C
Size 200µg
Description Rabbit Polyclonal antibody for CANX detection. Tested positive for IF, IF, IHC, IP, WB in Human, Mouse, Rat
Cite This Product Anti-Calnexin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03372)
Specificity Anti-Calnexin Antibody (A03372) reacts with Human, Mouse, Rat CANX, in native form and recombinant. Superfamily members of CANX are not reactive to A03372.
Host Rabbit
Contents/Buffer Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Form Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Immunogen Synthetic peptide corresponding to the sequence near the C-terminus of dog calnexin. The sequence is completely conserved in human, mouse and rat.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Calnexin Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

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Figure 1. IHC analysis of CANX using anti-CANX antibody (A03372).
CANX was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CANX Antibody (A03372) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 2. Western blot analysis of CANX using anti-CANX antibody (A03372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CANX antigen affinity purified polyclonal antibody (Catalog # A03372) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CANX.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P24643
Gene Name CANX

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.

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In stock
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A03372
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$386.00

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