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Product Info Summary
| SKU: | M03372 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CANX Antibody (C-Term)
SKU/Catalog Number
M03372
Size
50 µl
Description
Boster Bio Anti-CANX Antibody (C-Term) (Catalog # M03372). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CANX Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03372)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CANX antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 560-592 amino acids from human CANX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03372 is reactive to CANX in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
67568 Da
Background of Calnexin
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03372 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-CANX Antibody (C-Term) at 1:2000 dilution
Lane 1: PANC-1 lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse liver lysate
Lane 4: Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 68 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03372 staining CANX in human hepatocarcinoma sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing HeLa cells stained with M03372 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03372, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For CANX (Source: Uniprot.org, NCBI)
Gene Name
CANX
Full Name
Calnexin
Weight
67568 Da
Superfamily
calreticulin family
Alternative Names
Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX CANX CNX, IP90, P90 calnexin calnexin|epididymis secretory sperm binding protein|major histocompatibility complex class I -binding protein p88
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CANX, check out the CANX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CANX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CANX Antibody (C-Term) (M03372)
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