Product Info Summary
SKU: | M03372-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Calnexin CANX Rabbit Monoclonal Antibody
SKU/Catalog Number
M03372-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calnexin CANX Rabbit Monoclonal Antibody catalog # M03372-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calnexin CANX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03372-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calnexin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M03372-1 is reactive to CANX in Human, Rat
Applications
M03372-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
67.568kDa
Background of Calnexin
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Calnexin expression in HepG2 cell lysate.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CANX (Source: Uniprot.org, NCBI)
Gene Name
CANX
Full Name
Calnexin
Weight
67.568kDa
Superfamily
calreticulin family
Alternative Names
calnexin; CNX; IP90FLJ26570; Major histocompatibility complex class I antigen-binding protein p88; P90 CANX CNX, IP90, P90 calnexin calnexin|epididymis secretory sperm binding protein|major histocompatibility complex class I antigen-binding protein p88
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CANX, check out the CANX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CANX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calnexin CANX Rabbit Monoclonal Antibody (M03372-1)
Hello CJ!
M03372-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yu F,Liang M,Huang Y,Wu W,Zheng B,Chen C.Hypoxic tumor-derived exosomal miR-31-5p promotes lung adenocarcinoma metastasis by negatively regulating SATB2-reversed EMT and activating MEK/ERK signaling.J Exp Clin Cancer Res.2021 Jun 1;40(1):179.doi:10.1186/s13046-021-01979-7.PMID:34074322.
Species: Human,Mouse
M03372-1 usage in article: APP:WB, SAMPLE:LUAD CELL, DILUTION:NA
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Calnexin CANX Rabbit Monoclonal Antibody
Question
We are currently using anti-Calnexin Rabbit Monoclonal antibody M03372-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The anti-Calnexin Rabbit Monoclonal antibody (M03372-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-03