Product Info Summary
| SKU: | A03372S583 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Calnexin (phospho-S583) CANX Antibody
SKU/Catalog Number
A03372S583
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Calnexin (phospho-S583) CANX Antibody catalog # A03372S583. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calnexin (phospho-S583) CANX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03372S583)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Calnexin around the phosphorylation site of Serine 583.
Reactive Species
A03372S583 is reactive to CANX in Human, Mouse, Rat
Calculated molecular weight
67.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03372S583 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10000-1:50000
IHC: 1:50-1:100
0
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:10000 to 1:50000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of p-Calnexin (S583) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunoprecipitation of L02 whole cell lysate using Calnexin (phospho-S583) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5 ) .Lane 1 is 20% input. The western blot was probed using Calnexin (phospho-S583) pAb #BS4262.“↑”(supernatant); “↓”(deposition)
Specific Publications For Anti-Calnexin (phospho-S583) CANX Antibody (A03372S583)
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