CDA · Western blot design guide

CDA Western Blot Planning Guide

Plan a CDA Western blot around the catalog-observed 16.2 kDa band, image-backed A01578-1 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for CDA (CDA): expected band 16.2 kDa, antibody A01578-1, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

CDA Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	16.2 kDa
	Observed band	Not reported — verify product WB image
	Gel	15%
	Positive control ⓘ	Bone marrow
	Negative control ⓘ	Appendix

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	16.2 kDa
ⓘ	Localization	Not reported
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Sample controls for CDA Western blot

🧪Use Bone marrow as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.

Positive control: Bone marrow (Medium)

Negative control: Appendix (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for CDA

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Bone marrow	Reported tissue cells	Medium	Protein (HPA)	HPA →
Esophagus	Reported tissue cells	Low	Protein (HPA)	HPA →
Liver	Reported tissue cells	Low	Protein (HPA)	HPA →
Spleen	Reported tissue cells	Low	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Appendix	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Bronchus	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected CDA Western Blot Band Size?

Use the product-observed 16.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
16.2 kDa	Matches the authoritative product WB observation.
16.2 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected CDA appearancePlan around 16.2 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	16.2 kDa; use this as the primary experimental expectation.
Calculated mass	16.2 kDa from UniProt P32320; retain as context.
Gel selection	15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A01578-1.

Why is my band missing or off?
Situation	Likely cause	Next action
16.2 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt P32320 Human Protein Atlas product datasheet (A01578-1)

Experiment evidence

Western blot images showing CDA band

A01578-1 · CDA — observed 16.2 kDa WB validation

A01578-1 · CDAObserved 16.2 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated CDA Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Bone marrow
Gel %	15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, short transfer
Membrane	0.2 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A01578-1 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced CDA Western Blot Tips

Deeper troubleshooting and optimisation questions for CDA, answered from its protein features.

Which band should guide the blot?

Use 16.2 kDa, the observation attached to the authoritative A01578-1 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 16.2 kDa expectation.

Which positive control should I start with?

Start with Bone marrow, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Appendix as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for CDA Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A01578-1 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC8385558, PMC8056724, PMC12332198.

Boster reagents

CDA Western Blot Reagents

Human/Mouse/Rat-reactive CDA Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for CDA using A01578-1; observed band 16.2 kDa

Anti-CDA Antibody Picoband®

Cat # A01578-1

Reactivity: Human, Mouse, Rat

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.