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Product Info Summary
| SKU: | A01433-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-CDC25A(T507) Antibody
SKU/Catalog Number
A01433-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-CDC25A(T507) Antibody (Catalog # A01433-2). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Phospho-CDC25A(T507) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01433-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01433-2 is reactive to CDC25A in Human
Calculated molecular weight
59.1 kDa
Background of CDC25A
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01433-2 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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Fluorescent image of HeLa cells stained with Phospho-CDC25A (T507) Antibody. Phospho-CDC25A (T507) Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of extracts from Hela cells, untreated or treated with calyculin A, using Phospho-Cdc25A Antibody (T507).
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The anti-Phospho-CDC25A-T507 Pab is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Specific Publications For Anti-Phospho-CDC25A(T507) Antibody (A01433-2)
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