This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CDC6 · Western blot design guide
CDC6 Western Blot Planning Guide
Plan a CDC6 Western blot around the catalog-observed 62.7 kDa band, image-backed PA2199 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CDC6 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 62.7 kDa observed | |
| Observed band | 62.7 kDa observed | |
| Gel | 12-15% | |
| Positive control | Testis | |
| Negative control | Oral mucosa |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 62.7 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Rat |
Section 1
Real Curated CDC6 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2199 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CDC6 Western Blot Band Size?
Use the product-observed 62.7 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 62.7 kDa | Matches the authoritative product WB observation. |
| 62.7 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CDC6 appearancePlan around 62.7 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 62.7 kDa; use this as the primary experimental expectation. |
| Calculated mass | 62.7 kDa from UniProt Q99741; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2199. |
| Situation | Likely cause | Next action |
|---|---|---|
| 62.7 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CDC6 band
PA2199 · CDC6
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CDC6 Western blot
🧪Use Testis as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Testis (High)
Negative control: Oral mucosa (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CDC6
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Esophagus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Skeletal muscle | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced CDC6 Western Blot Tips
Deeper troubleshooting and optimisation questions for CDC6, answered from its protein features.
Which band should guide the blot?
Use 62.7 kDa, the observation attached to the authoritative PA2199 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 62.7 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Oral mucosa as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CDC6 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2199 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CDC6 Western Blot Reagents
Human/Rat-reactive CDC6 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Cdc6 Antibody Picoband®
Cat # PA2199
Real WB data
Anti-Cdc6 Antibody Picoband®
Cat # PB9531
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q99741
- Human Protein Atlas — CDC6
- PA2199 product record
- PMC1456921 — The functional role of Cdc6 in S-G2/M in mammalian cells (EMBO reports, 2006)
- PMC6614418 — mTOR Signaling Upregulates CDC6 via Suppressing miR-3178 and Promotes the Loading of DNA Replication Helicase (Scientific reports, 2019)
- PMC1951745 — Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage (Molecular biology of the cell, 2007)