CHD8 Antibodies

AND CHD8 ELISA kits, CHD8 Proteins

Many biological assays use antibodies to detect CHD8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CHD8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CHD8 antibodies...

We validate the specificity of these antibodies to CHD8 by testing them on tissues known to express CHD8 positively and negatively. Browse below to find the CHD8 antibody that suites your experiment. We have 1 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for CHD8? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CHD8 Western Blot Guide

CHD8 Infographic by Boster Bio

All CHD8 Products

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Anti-CHD8 Antibody Picoband®

Western blot analysis of CHD8 using anti-CHD8 antibody (A01346-1)

IHC analysis of CHD8 using anti-CHD8 antibody (A01346-1)

Flow Cytometry analysis of SH-SY5Y cells using anti-CHD8 antibody (A01346-1)

Human

ELISA, Flow Cytometry, IHC, WB

$370
Cat # A01346-1
100 µg/vial

CHD8 Overview

Facts about Chromodomain-helicase-DNA-binding protein 8.

CHD8 3D structure. Source: alphafold

Chromodomain-helicase-DNA-binding protein 8 (CHD8)

DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruitment histone H1 to target genes.

Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes.

Gene Information

Human
Gene Name:	CHD8
Uniprot:	Q9HCK8
Entrez:	57680

Family

Belongs to:

SNF2/RAD54 helicase family

Alternative Names

ATP-dependent helicase CHD8; CHD-8; chromodomain helicase DNA binding protein 8; DKFZp686N17164; duplin; EC 3.6.1; EC 3.6.1.7; EC 3.6.4.12; Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8; HELSNF1; KIAA1564axis duplication inhibitor

Molecular Weight

Mass (kDA):
290.519 kDA

Genomic Context


Human

Location:	14q11.2
Sequence:	14; NC_000014.9 (21385199..21456123, complement)

Subcellular Localizations

Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.

Diseases

Autistic Disorder
Autism Spectrum Disorders
Malignant Neoplasms
Charge Syndrome
Congenital Heart Defects
Developmental Disabilities
Child Development Disorders, Pervasive
Tuberculosis, Pulmonary
Stomach Neoplasms
Congenital Ocular Coloboma (disorder)
Malignant Neoplasm Of Breast
Spastic Paraplegia, Hereditary

Macrocephaly
Paraplegia
Neoplasms
Chromosome Breakage
Mammary Neoplasms
Colorectal Cancer

Interacting Proteins

ASH2L
CHD7
RBBP5
WDR5
ZBTB7A

Pathways

Chromatin Remodeling
Atp-dependent Chromatin Remodeling
Methylation
Induction Of Apoptosis
Cell Cycle
Pathogenesis
Cell Proliferation
Histone Acetylation

Histone Modification
Chromatin Modification
Rna Interference

PTMs

Methylation

Phosphorylation

Acetylation

Research Areas

Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CHD8

References

PMID: 15960975 by Dou Y., et al. Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.

PMID: 16949368 by Ishihara K., et al. CTCF-dependent chromatin insulator is linked to epigenetic remodeling.