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- Table of Contents
CLC · Western blot design guide
CLC Western Blot Planning Guide
Plan a CLC Western blot around the catalog-observed 16.5 kDa band, image-backed A01350 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CLC Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 16.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 15% | |
| Positive control | Spleen | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 16.5 kDa |
| ⓘ | Localization | Cytoplasm, cytosol / Cytoplasmic granule |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated CLC Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bone marrow |
| Gel % | 15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.2 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01350 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CLC Western Blot Band Size?
Use the product-observed 16.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 16.5 kDa | Matches the authoritative product WB observation. |
| 16.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CLC appearancePlan around 16.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 16.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 16.5 kDa from UniProt Q05315; retain as context. |
| Gel selection | 15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01350. |
| Situation | Likely cause | Next action |
|---|---|---|
| 16.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CLC band
A01350 · CLC
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CLC Western blot
🧪Use Spleen as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Spleen (Medium)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CLC
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Spleen | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced CLC Western Blot Tips
Deeper troubleshooting and optimisation questions for CLC, answered from its protein features.
Which band should guide the blot?
Use 16.5 kDa, the observation attached to the authoritative A01350 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 16.5 kDa expectation.
Which positive control should I start with?
Start with Spleen, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CLC Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01350 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CLC Western Blot Reagents
Human-reactive CLC Western blot reagents with authoritative product imagery.
Real WB data
Anti-Galectin 10/CLC Antibody Picoband®
Cat # A01350
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q05315
- Human Protein Atlas — CLC
- A01350 product record
- PMC11382115 — Serum Galectin-10: A biomarker for persistent airflow limitation in adult asthmatics (The World Allergy Organization journal, 2024)
