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- Table of Contents
CRB1 · Western blot design guide
CRB1 Western Blot Planning Guide
Plan a CRB1 Western blot around the catalog-observed 154.2 kDa band, image-backed A01499-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CRB1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 154.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Retina | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 154.2 kDa |
| ⓘ | Localization | Apical cell membrane / Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for CRB1 Western blot
🧪Use Retina as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Retina (Medium)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CRB1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Retina | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 1
What Is the Expected CRB1 Western Blot Band Size?
Use the product-observed 154.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 154.2 kDa | Matches the authoritative product WB observation. |
| 154.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CRB1 appearancePlan around 154.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 154.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 154.2 kDa from UniProt P82279; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01499-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 154.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CRB1 band
A01499-1 · CRB1Observed 154.2 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated CRB1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Retina |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01499-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced CRB1 Western Blot Tips
Deeper troubleshooting and optimisation questions for CRB1, answered from its protein features.
Which band should guide the blot?
Use 154.2 kDa, the observation attached to the authoritative A01499-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 154.2 kDa expectation.
Which positive control should I start with?
Start with Retina, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CRB1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01499-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC4468907, PMC7335077.
Boster reagents
CRB1 Western Blot Reagents
Human/Mouse/Rat-reactive CRB1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-CRB1 Antibody Picoband®
Cat # A01499-1
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P82279
- Human Protein Atlas — CRB1
- A01499-1 product record
- PMC4468907 — A network comprising short and long noncoding RNAs and RNA helicase controls mouse retina architecture.
- PMC7335077 — Comprehensive identification of mRNA isoforms reveals the diversity of neural cell-surface molecules with roles in retinal development and disease.