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- Table of Contents
CRTC3 · Western blot design guide
CRTC3 Western Blot Planning Guide
Plan a CRTC3 Western blot around the catalog-observed 67 kDa band, image-backed A04568-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CRTC3 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 67 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Ovary | |
| Negative control | Duodenum |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 67 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated CRTC3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A04568-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CRTC3 Western Blot Band Size?
Use the product-observed 67 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 67 kDa | Matches the authoritative product WB observation. |
| 67 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CRTC3 appearancePlan around 67 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 67 kDa; use this as the primary experimental expectation. |
| Calculated mass | 67 kDa from UniProt Q6UUV7; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A04568-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 67 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CRTC3 band
A04568-2 · CRTC3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CRTC3 Western blot
🧪Use Ovary as the first positive-control candidate and Duodenum as the HPA Not detected negative candidate.
Positive control: Ovary (High)
Negative control: Duodenum (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CRTC3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Ovary | Reported tissue cells | High | Protein (HPA) | HPA → |
| Salivary gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced CRTC3 Western Blot Tips
Deeper troubleshooting and optimisation questions for CRTC3, answered from its protein features.
Which band should guide the blot?
Use 67 kDa, the observation attached to the authoritative A04568-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 67 kDa expectation.
Which positive control should I start with?
Start with Ovary, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Duodenum as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CRTC3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A04568-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CRTC3 Western Blot Reagents
Human-reactive CRTC3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-CRTC3 Antibody Picoband®
Cat # A04568-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q6UUV7
- Human Protein Atlas — CRTC3
- A04568-2 product record
- PMC6899503 — Involvement of CREB-regulated transcription coactivators (CRTC) in transcriptional activation of steroidogenic acute regulatory protein (Star) by ACTH (Molecular and cellular endocrinology, 2020)
- PMC6317279 — Mitogenic Signals Stimulate the CREB Coactivator CRTC3 through PP2A Recruitment (iScience, 2019)
- PMC3025711 — CRTC3 links catecholamine signalling to energy balance (Nature, 2010)