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CYP27B1 Antibodies

AND CYP27B1 ELISA kits, CYP27B1 Proteins

Many biological assays use antibodies to detect CYP27B1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CYP27B1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CYP27B1 antibodies...

We validate the specificity of these antibodies to CYP27B1 by testing them on tissues known to express CYP27B1 positively and negatively. Browse below to find the CYP27B1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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CYP27B1 Infographic by Boster Bio

CYP27B1 protein infographic

All CYP27B1 Products

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Anti-CYP27B1 Antibody Picoband®

1 Citations 4 Q&As

Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548)

Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548)

IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548)

IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548)

Human, Mouse, Rat

IHC, WB

$370
Cat # PB9548
100 μg/vial

Anti-CYP27B1 Antibody (C-term)

Anti-CYP27B1 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 µg per lane

Anti-CYP27B1 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 µg per lane

Human

WB

$299
Cat # M01370
50 µl

CYP27B1 (NM_000785) Human Over-expression Lysate

CYP27B1 Human Over-expression Lysates NM_000785

CYP27B1 Human Over-expression Lysates NM_000785

Human

$960
Cat # LS001594
20 µg, 100 µg

Anti-CYP27B1 Antibody Picoband®

Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (A01370)

Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (A01370)

Human

ELISA, WB

$370
Cat # A01370
100 µg/vial

CYP27B1 Overview

Facts about 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial.

CYP27B1 3D structure

CYP27B1 3D structure. Source: alphafold

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1)

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) into 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1- alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3.

Plays an essential role in normal bone growth, calcium metabolism, and tissue differentiation. .

Gene Information

Human
Gene Name:	CYP27B1
Uniprot:	O15528
Entrez:	1594

Gene Information

Family

Belongs to:

cytochrome P450 family

Family

Alternative Names

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Molecular Weight

Mass (kDA):
56.504 kDA

Molecular Weight

Genomic Context


Human

Location:	12q14.1
Sequence:	12; NC_000012.12 (57762334..57767078, complement)

Tissue Specificity

Kidney.

Subcellular Localizations

Mitochondrion membrane.

Subcellular Localization

Diseases

Rickets vitamin D-dependent 1A (VDDR1A)

Disease

Bosterbio | ELISA Kits, Antibodies, Antibody Company

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP27B1

References

PMID: 9428799 by Fu G.K., et al. Complete structure of the human gene for the vitamin D 1alpha- hydroxylase, P450c1alpha.

PMID: 9344864 by Monkawa T., et al. Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1 alpha-hydroxylase.