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- Table of Contents
Protein cereblon Antibodies
AND CRBN ELISA kits, Protein cereblon Proteins
Many biological assays use antibodies to detect Protein cereblon, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Protein cereblon in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Protein cereblon antibodies...
Designing a Western blot for Protein cereblon? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CRBN Western Blot GuideCRBN Infographic by Boster Bio
All CRBN Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, ICC, WB
$370
Cat # A00983-1
100 μg/vial
Cat # A00983-1
100 μg/vial
Human, Mouse, Rat
ELISA, IF, IHC-P, WB
$481
Cat # A00983
0.1 mg
Cat # A00983
0.1 mg
CRBN Overview
Facts about Protein cereblon.
Protein cereblon (CRBN)
Substrate recognition component of a DCX (DDB1-CUL4-X- box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Regular degradation of key regulatory proteins is necessary for normal limb outgrowth and expression of the fibroblast growth factor FGF8.
May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
Gene Information
| Human | |
|---|---|
| Gene Name: | CRBN |
| Uniprot: | Q96SW2 |
| Entrez: | 51185 |
Family
| Belongs to: |
|---|
| CRBN family |
Alternative Names
Cereblon; CRBN; DKFZp781K0715; MGC27358; MRT2; MRT2A; non-syndromic, autosomal recessive, 2A; protein cereblon; Protein X 0001
Molecular Weight
Mass (kDA):
50.546 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p26.2 |
| Sequence: | 3; NC_000003.12 (3148490..3179717, complement) |
Tissue Specificity
Widely expressed. Highly expressed in brain.
Subcellular Localizations
Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
Diseases
- Multiple Myeloma
- Teratogenic Effect
- Malignant Neoplasms
- Congenital Abnormality
- Leprosy
- Mild Mental Retardation
- Limb Deformities, Congenital
- Microcephaly
- Hematologic Neoplasms
- Monosomy
- Blepharoptosis
- Neuroblastoma
Interacting Proteins
- CSNK1A1
- DDB1
- PAK5
- RBPMS
Pathways
- Neuroprotection
- Cytokine Production
- Membrane Depolarization
- Gene Silencing
- Brain Development
- Immune Response
- Glycosylation
- Associative Learning
PTMs
- Phosphorylation
- Glycosylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CRBN
bosterbio.com/bosterbio-gene-info-cards/CRBN
References
PMID: 15557513 by Higgins J.J., et al. A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation.
PMID: 17380424 by Xin W., et al. Primary function analysis of human mental retardation related gene CRBN.