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- Table of Contents
CRBN antibody (A00983) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of CRBN in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with A00983 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with A00983 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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Anti-Protein cereblon CRBN Antibody
CRBN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Protein cereblon CRBN Antibody
View all CRBN Antibodies
View all CRBN Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
CRBN antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
CRBN antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN. The immunogen is located within the last 50 amino acids of CRBN.
Cross-Reactivity
CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
50.5 kDa
Background of CRBN
CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).
Cite This Product
Anti-Protein cereblon CRBN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00983)
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Product Promise
We promise Anti-Protein cereblon CRBN Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00983
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026