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Product Info Summary
| SKU: | A00983 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein cereblon CRBN Antibody
SKU/Catalog Number
A00983
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein cereblon CRBN Antibody (Catalog # A00983). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CRBN antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein cereblon CRBN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00983)
Host
Rabbit
Contents
CRBN antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN. The immunogen is located within the last 50 amino acids of CRBN.
Cross-reactivity
CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Reactive Species
A00983 is reactive to CRBN in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.5 kDa
Background of CRBN
CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00983 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunofluorescence Validation of CRBN in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with A00983 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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WB Validation in Human Testis
Loading: 15 μg of human testis lysate
Antibodies: CRBN A00983, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane A: 0.5 μg/mL
Lane B: 1 μg/mL
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WB Validation in Human Cells
Loading: 15 μg of cell lysate
Antibodies: CRBN A00983, 1 μg/mL,1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Mouse Tissues
Loading: 15 μg of mouse tissue lysate
Antibodies: CRBN A00983, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Rat Tissues
Loading: 15 μg of rat tissue lysate
Antibodies: CRBN A00983, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Specific Publications For Anti-Protein cereblon CRBN Antibody (A00983)
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