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- Table of Contents
DBH · Western blot design guide
DBH Western Blot Planning Guide
Plan a DBH Western blot around the catalog-observed 69.1 kDa band, image-backed A01110 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
DBH Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 69.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Adrenal gland | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 69.1 kDa |
| ⓘ | Localization | Cytoplasmic vesicle, secretory vesicle lumen / Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated DBH Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01110 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected DBH Western Blot Band Size?
Use the product-observed 69.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 69.1 kDa | Matches the authoritative product WB observation. |
| 69.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected DBH appearancePlan around 69.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 69.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 69.1 kDa from UniProt P09172; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01110. |
| Situation | Likely cause | Next action |
|---|---|---|
| 69.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing DBH band
A01110 · DBH
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for DBH Western blot
🧪Use Adrenal gland as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Adrenal gland (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for DBH
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Section 3
Advanced DBH Western Blot Tips
Deeper troubleshooting and optimisation questions for DBH, answered from its protein features.
Which band should guide the blot?
Use 69.1 kDa, the observation attached to the authoritative A01110 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 69.1 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for DBH Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01110 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
DBH Western Blot Reagents
Human/Mouse/Rat-reactive DBH Western blot reagents with authoritative product imagery.
Real WB data
Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband®
Cat # A01110
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P09172
- Human Protein Atlas — DBH
- A01110 product record
- PMC3874437 — Electroacupuncture Analgesia Is Improved by Adenoviral Gene Transfer of Dopamine Beta-hydroxylase into the Hypothalamus of Rats (The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 2013)
- PMC9338777 — Effects of chronic social defeat on expression of dopamine β-hydroxylase in rat brains (Synapse (New York, N.Y.), 2013)
- PMC9339151 — Effects of transcription factors Phox2 on expression of norepinephrine transporter and dopamine beta-hydroxylase in SK-N-BE(2)C cells (Journal of neurochemistry, 2009)