DGKE Antibodies

AND DGKE ELISA kits, DGKE Proteins

Many biological assays use antibodies to detect DGKE, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DGKE in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DGKE antibodies...

We validate the specificity of these antibodies to DGKE by testing them on tissues known to express DGKE positively and negatively. Browse below to find the DGKE antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for DGKE? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the DGKE IHC Guide

DGKE Infographic by Boster Bio

All DGKE Products

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Product

Figures

Specificity

Applications

Price

Anti-DGK-Epsilon (S194) DGKE Antibody

Immunohistochemistry (IHC) analyzes of DGK-ε(S194) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50

Human, Mouse, Rat

IHC

$405
Cat # A06615S194
100ul

DGKE Colorimetric Cell-Based ELISA

Western blot analysis of extracts from K562 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1740
1 kit, containing one 96-well plate and all necessary reagents

Anti-DGK-Epsilon DGKE Antibody

Western blot analysis of extracts of various cell lines, using DGKE antibody at 1:1000 dilution

Human, Mouse

$405
Cat # A06615-1
100ul

Anti-DGK-epsilon Antibody

Western blot analysis of lysates from K562 cells, using DGKE Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A06615-2
100ul

DGKE (NM_003647) Human Recombinant Protein

Coomassie blue staining of purified DGKE protein

$1249
Cat # PROTP52429
20 µg

DGKE Overview

Facts about Diacylglycerol kinase epsilon.

DGKE 3D structure. Source: alphafold

Diacylglycerol kinase epsilon (DGKE)

Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may bring about the synthesis of phospholipids with defined fatty acid composition.

Gene Information

Human
Gene Name:	DGKE
Uniprot:	P52429
Entrez:	8526

Family

Belongs to:

eukaryotic diacylglycerol kinase family

Alternative Names

DAG kinase epsilon; DAGK5; DAGK6; DGK; DGKE; DGKepsilon; DGK-epsilon; diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon (64kD); diacylglycerol kinase, epsilon 64kDa; Diglyceride kinase epsilon; EC 2.7.1.107

Molecular Weight

Mass (kDA):
63.927 kDA

Genomic Context


Human

Location:	17q22
Sequence:	17; NC_000017.11 (56834131..56869567)

Tissue Specificity

Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).

Subcellular Localizations

Membrane; Multi-pass membrane protein. Membrane. Cytoplasm.

Diseases

Cardiovascular Diseases
Depressive Disorder
Diabetes Mellitus
Dysequilibrium Syndrome

Pathways

Regulation Of Gene Expression

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DGKE

References

PMID: 8626589 by Tang W., et al. Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates.

PMID: 10571048 by Tang W., et al. Characterization of the human diacylglycerol kinase epsilon gene and its assessment as a candidate for inherited retinitis pigmentosa.