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- Table of Contents
DHPS · Western blot design guide
DHPS Western Blot Planning Guide
Plan a DHPS Western blot around the catalog-observed 41 kDa band, image-backed A02106-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
DHPS Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 41 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Gallbladder | |
| Negative control | Cerebral cortex |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 41 kDa |
| ⓘ | Localization | Not reported |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for DHPS Western blot
🧪Use Gallbladder as the first positive-control candidate and Cerebral cortex as the HPA Not detected negative candidate.
Positive control: Gallbladder (High)
Negative control: Cerebral cortex (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for DHPS
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 1
What Is the Expected DHPS Western Blot Band Size?
Use the product-observed 41 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 41 kDa | Matches the authoritative product WB observation. |
| 41 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected DHPS appearancePlan around 41 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 41 kDa; use this as the primary experimental expectation. |
| Calculated mass | 41 kDa from UniProt P49366; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02106-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 41 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing DHPS band
A02106-2 · DHPSObserved 41 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated DHPS Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02106-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced DHPS Western Blot Tips
Deeper troubleshooting and optimisation questions for DHPS, answered from its protein features.
Which band should guide the blot?
Use 41 kDa, the observation attached to the authoritative A02106-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 41 kDa expectation.
Which positive control should I start with?
Start with Gallbladder, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Cerebral cortex as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for DHPS Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02106-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC10275725, PMC11704301, PMC7812972.
Boster reagents
DHPS Western Blot Reagents
Human/Mouse/Rat-reactive DHPS Western blot reagents with authoritative product imagery.
Real WB data
Anti-DHPS/DHS Antibody Picoband®
Cat # A02106-2
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P49366
- Human Protein Atlas — DHPS
- A02106-2 product record
- PMC10275725 — Deoxyhypusine synthase mutations alter the post-translational modification of eukaryotic initiation factor 5A resulting in impaired human and mouse neural homeostasis.
- PMC11704301 — Knockout or inhibition of DHPS suppresses ovarian tumor growth and metastasis by attenuating the TGFβ pathway.
- PMC7812972 — Hypusination Orchestrates the Antimicrobial Response of Macrophages.