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DUSP10 / Dual specificity protein phosphatase 10 · IHC design guide
Design Immunohistochemistry for DUSP10
Plan DUSP10 paraffin-section IHC around often granular cytoplasmic staining, with high signals reported in several glandular cell populations (HPA tissue IHC). Start the catalog antibody at 1:50 (datasheet A05412) and assess staining against appropriate controls (standard IHC practice).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
DUSP10 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic in tissue (HPA tissue IHC); nuclear possible (UniProt) | |
| Staining pattern | Often granular cytoplasm in glandular cells (HPA tissue IHC) | |
| Antigen retrieval | Tris-EDTA pH 9.0 HIER, heat-mediated (datasheet A05412) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | None in HPA (detected in all 45 tissues); use no-primary + isotype controls |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across sections (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A05412) | |
| Caveat | Staining and RNA show medium consistency (HPA tissue IHC) | |
| Regulation | Staining regulation not specified (UniProt) | |
| Isoform / epitope | 2 isoforms; epitope coverage needs checking (UniProt) |
Section 1
Recommended DUSP10 IHC & IF Protocols
The catalog antibody protocol is accompanied by published DUSP10 IHC methods for breast tissue (PMC8389698) and lung tumors (PMC10475826).
| Sample | Paraffin-embedded human meningioma tissue; fixative not specified (datasheet A05412) |
| Fixation | Image fixative and duration unreported (datasheet A05412); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Tris-EDTA pH 9.0 (datasheet A05412); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit anti-DUSP10, 1:50 recommended; image 1:200 (datasheet A05412) |
| Primary incubation | Overnight at 4 °C (standard) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | DUSP10-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: General cytoplasmic expression, often with a granular a pattern. No signal in the no-primary control. |
💡Decision noteStart with Tris-EDTA pH 9.0 heat retrieval (datasheet A05412); the lung tumor protocol also used this buffer (PMC10475826).
Section 2
What Is the Expected DUSP10 Staining Pattern?
DUSP10 is a cytoplasmic and nuclear phosphatase with no transmembrane segment (UniProt Q9Y6W6). In paraffin sections, expect mainly cytoplasmic staining, often granular, in glandular cells and cortical neurons reported as high by HPA (HPA: tissue IHC). HPA rates its tissue staining Approved, with medium consistency between staining and RNA data (HPA: tissue IHC). Interpret intensity within the identified cell type rather than across the whole section (general IHC practice).
| Granular cytoplasmic staining in glandular cells of colon, breast, or adrenal gland, or in cerebral cortical neurons (HPA: tissue IHC). | This fits HPA's general cytoplasmic profile and its High calls for those cells (HPA: tissue IHC). Nuclear staining can coexist: UniProt lists both compartments (UniProt Q9Y6W6). |
| A sharp membrane rim or extracellular deposit dominates the signal (general IHC observation). | That is discordant with HPA's cytoplasmic pattern and UniProt's cytoplasmic/nuclear location and lack of a transmembrane segment (HPA: tissue IHC; UniProt Q9Y6W6). Check controls before assigning it to DUSP10 (general IHC practice). |
| Fibroblasts stain strongly while nearby glandular cells remain weak (general IHC observation). | HPA reports Low staining in soft tissue fibroblasts but High staining in several glandular cell populations (HPA: tissue IHC). Consider antibody cross-reactivity or endogenous chromogen activity; one section cannot distinguish them (general IHC practice). |
| Broad, diffuse color obscures cell boundaries and appears across unrelated structures (general IHC observation). | The field cannot be scored reliably for HPA's cellular cytoplasmic pattern (HPA: tissue IHC). Review the no-primary control, blocking, washes, and detection conditions (general IHC practice). |
| No stain appears in glandular cells of a colon section run as a positive reference (HPA: High in colon glandular cells). | A blank reference raises concern about assay performance; it does not establish absent DUSP10 (general IHC practice). Check tissue integrity, retrieval, antibody dilution, and detection alongside controls (general IHC practice). |
💡Expected DUSP10 appearanceCall a result positive when interpretable cytoplasmic staining, often granular, occurs in HPA High glandular cells or cortical neurons; nuclear signal is compatible, while dominant membrane or extracellular color is suspect (HPA: tissue IHC; UniProt Q9Y6W6).
| Compartment | HPA describes general cytoplasmic staining, often granular; UniProt also lists the nucleus (HPA: tissue IHC; UniProt Q9Y6W6). Score each compartment separately when both are visible (general IHC practice). |
| Reference cell type | HPA calls glandular cells High in adrenal gland, breast, colon, and other listed tissues, and cortical neurons High; its listed fibroblasts and adipocytes are Low (HPA: tissue IHC). Low is not a negative control (general IHC practice). |
| Evidence strength | HPA calls tissue staining Approved with medium staining–RNA consistency; HPA016758 is IHC Approved (HPA: tissue IHC; HPA: antibody record). Treat an unexpected pattern as a finding to verify, not a validated new localization (general IHC practice). |
| Protein form | UniProt lists two isoforms, one chain spanning residues 1–482, no signal peptide or propeptide, and no recorded glycosylation sites (UniProt Q9Y6W6). These annotations alone do not identify which isoform an IHC antibody detects (general IHC interpretation). |
| IF/ICC expectation? | HPA reports supported nucleoplasm and cytosol localization in ICC-IF, with images from A-431, U-251MG, and U2OS (HPA: subcellular; HPA: antibody record). This supports a compartment comparison, not an IHC intensity prediction (general assay interpretation). |
| Chromogenic detection | Endogenous enzyme activity can produce color independently of primary-antibody binding (general IHC practice). Use the detection system's appropriate control before attributing unusual color to DUSP10 (general IHC practice). |
| Situation | Likely cause | Next action |
|---|---|---|
| High-reference tissue is blank (HPA: High in colon glandular cells). | The primary or detection step may have failed, or retrieval may be unsuitable for this assay (general IHC practice). | Run a known-positive section beside the sample; verify reagent sequence, dilution, retrieval conditions, and detector function (general IHC practice). No DUSP10-specific retrieval condition is supplied. |
| Color is widespread, including outside intact cells (general IHC observation). | Nonspecific background, incomplete washing, or endogenous detection activity may contribute (general IHC practice). | Compare a no-primary control, review blocking and washes, and check endogenous-activity control appropriate to the chromogen system (general IHC practice). |
| Membrane staining dominates (general IHC observation). | This conflicts with the reported cytoplasmic/nuclear location and lack of a transmembrane segment (UniProt Q9Y6W6). | Check the no-primary control and compare staining in an HPA High cell population before interpreting the rim as DUSP10 (HPA: tissue IHC; general IHC practice). |
| Fibroblasts outshine glandular cells (general IHC observation). | HPA lists soft tissue fibroblasts as Low and several glandular populations as High; cross-reactivity or endogenous color is possible (HPA: tissue IHC; general IHC practice). | Confirm cell identity, inspect the no-primary control, and repeat with a validated antibody or independent specificity evidence if the discrepancy persists (general IHC practice). |
| Nuclear staining appears alongside cytoplasm (general IHC observation). | Both compartments are reported for DUSP10; HPA ICC-IF supports nucleoplasm and cytosol (UniProt Q9Y6W6; HPA: subcellular). | Record nuclear and cytoplasmic scores separately and check that nuclear color exceeds background controls (general IHC practice). |
| A Low-reference population has faint staining (HPA: Low in adipocytes, fibroblasts, and other listed cells). | Low HPA staining is not an absence claim, and intensity can depend on assay conditions (HPA: tissue IHC; general IHC practice). | Compare matched positive and no-primary controls; avoid calling faint Low-reference staining a failed negative control (general IHC practice). |
Experiment evidence
IHC & IF images showing DUSP10 staining
A05412 · IHCImmunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000143507-DUSP10/tissue/cerebral+cortex#imid_5648024
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000143507-DUSP10/tissue/colon#imid_5647950
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000143507-DUSP10/tissue/liver#imid_5647943
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000143507-DUSP10/tissue/kidney#imid_5647952
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA016758; green = DUSP10, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA016758; green = DUSP10, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for DUSP10 IHC & IF
🧪Run colon first and assess staining in its glandular cells (HPA: High in colon glandular cells). HPA detects DUSP10 in all 45 scored tissues, so there is no supported negative tissue; use no-primary and isotype controls, and treat staining in non-glandular cells as an observation rather than assuming those cells are internal negatives (HPA: no negative tissue rows).
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: None in HPA: DUSP10 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead.
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show DUSP10 in A-431, U-251MG, U2OS, with annotated localisation: Nucleoplasm (supported), Cytosol (supported) (HPA subcellular).
Technical controls: Include a secondary-only slide and a control antibody matched to the primary antibody’s host species and clonality; a matched DUSP10 knockout specimen or validated peptide block would provide a biological specificity control (standard IHC practice). For chromogenic colon IHC, quench endogenous peroxidase and check the control slides for background staining (standard IHC practice).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected A05412 paraffin-section caption does not state the fixative (selected A05412 caption: fixative unreported). The caption uses Tris-EDTA at pH 9.0 for retrieval and an antibody dilution of 1:200 overnight at 4°C, providing an IHC starting point without establishing a retrieval dependency (selected A05412 caption). The supplied evidence does not establish that frozen sections or IF are easier; for colon sections, evaluate luminal and epithelial background on the control slides before scoring glandular staining (HPA: High in colon glandular cells; standard IHC practice).
HPA tissue IHC evidence for DUSP10
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Approved — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Breast | Glandular cells | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
| Colon | Glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | Glandular cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| None in HPA: DUSP10 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead. | ||||
Section 3
Advanced DUSP10 IHC Tips
These checks help distinguish DUSP10 staining from processing and detection artefacts in chromogenic IHC of paraffin sections.
Which retrieval condition should I start with for weak DUSP10 staining?
Use heat-mediated Tris-EDTA at pH 9.0 for antigen retrieval before chromogenic DUSP10 IHC on paraffin sections (datasheet A05412). The selected meningioma image used primary antibody at 1:200 at 4°C overnight, followed by secondary antibody at 1:200 for 45 min at room temperature (caption A05412). Compare heating and cooling conditions on matched sections while keeping section thickness and detection consistent (standard IHC practice). If staining remains weak, test another buffer only as a controlled fallback alongside the stated pH 9.0 condition (standard IHC practice). Judge retrieval by cellular staining and preserved morphology, not total brown intensity alone (standard IHC practice).
Could fixation explain inconsistent DUSP10 staining between specimens?
The selected caption identifies paraffin-embedded meningioma but gives no fixative, so DUSP10-specific fixation sensitivity is unknown (caption A05412). Do not infer a fixation response from tissue staining patterns or cytoplasmic and nuclear localization (HPA tissue IHC; UniProt Q9Y6W6). Record fixative, fixation duration, delay before fixation, and processing for each specimen, then compare matched sections under identical retrieval and detection conditions (standard IHC practice). If signal varies, inspect preservation, edge staining, and morphology before changing antibody concentration; the selected primary dilution was 1:200 (caption A05412; standard IHC practice). Include a control section processed alongside each staining batch (standard IHC practice).
How should I interpret cytoplasmic versus nuclear DUSP10 staining?
Expect predominantly cytoplasmic staining, sometimes granular, in tissue IHC (HPA tissue IHC); nuclear localization is also documented for DUSP10 (UniProt Q9Y6W6). Supported nucleoplasmic and cytosolic localization from cell imaging makes either compartment plausible, but does not establish a fixed nuclear-to-cytoplasmic ratio for paraffin sections (HPA subcellular). Score nuclear and cytoplasmic signal separately within identified cells, and keep counterstain and detection conditions consistent across sections (standard IHC practice). Compare compartments with a control section and check whether apparent nuclear staining follows tissue folds or damaged edges (standard IHC practice). Diffuse extracellular deposit does not match the documented intracellular localization (UniProt Q9Y6W6; HPA subcellular).
Can this antibody distinguish DUSP10 isoforms or masked epitopes?
DUSP10 has 2 reported isoforms, while the supplied product caption does not identify the recognized epitope or establish isoform selectivity (UniProt Q9Y6W6; caption A05412). Its rhodanese domain spans residues 168–285 and its phosphatase domain spans 321–464, but neither span identifies this antibody's binding site (UniProt Q9Y6W6). Check the antibody's epitope documentation before assigning isoform-specific staining, and avoid equating altered intensity with isoform switching (standard IHC practice). Compare matched sections using the stated Tris-EDTA pH 9.0 retrieval when evaluating possible epitope masking (datasheet A05412; standard IHC practice). Interpret any unresolved staining as DUSP10 signal without an isoform assignment.
How can I cross-check DUSP10 IHC with multiplex immunofluorescence?
Use IF as a separate validation experiment and pair DUSP10 with a marker for the expected cell population; glandular cells in several listed tissues show high tissue IHC staining (HPA tissue IHC). Select spectrally separated fluorophores after examining unstained tissue for autofluorescence, and include single-label controls to assess channel bleed-through (standard IF practice). DUSP10 is documented in cytosol and nucleoplasm and has no transmembrane segment, so permeabilisation should permit access to intracellular epitopes (HPA subcellular; UniProt Q9Y6W6; standard IF practice). Compare cytosolic and nuclear patterns within the same marked cells rather than relying on merged color alone (standard IF practice). The paraffin IHC caption supplies no IF fixation or permeabilisation condition (caption A05412).
What should I check when brown signal spreads beyond DUSP10-positive cells?
First inspect a no-primary control for secondary or chromogen signal, and use a peroxidase block when applying a peroxidase-based chromogenic workflow (standard IHC practice). Check whether staining follows folds, cut edges, trapped reagent, or necrotic material rather than intact cells (standard IHC practice). DUSP10 has documented cytoplasmic and nuclear localization, while tissue IHC commonly shows a granular cytoplasmic pattern (UniProt Q9Y6W6; HPA tissue IHC). If background rises with signal, compare matched sections at the selected primary dilution of 1:200 before changing detection conditions (caption A05412; standard IHC practice). Assess background separately from staining in morphologically intact cells (standard IHC practice).
How should I quantify DUSP10 staining across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the scored cell population and compartment before reviewing slides; tissue IHC reports high glandular-cell staining in several organs and generally cytoplasmic expression (HPA tissue IHC). Report the percentage of positive cells and staining intensity, or calculate an H-score from percentages at intensity grades 0–3 (standard IHC practice). Score nuclear and cytoplasmic staining separately where both are present, since both locations are documented for DUSP10 (UniProt Q9Y6W6; HPA subcellular). Normalize counts to eligible intact cells, or area-based counts to tissue area in mm², while excluding folds and necrosis by the same rules throughout (standard IHC practice). Keep acquisition, counterstain, and scoring thresholds consistent (standard IHC practice).
When is an apparent DUSP10-positive area likely to be artefact?
Treat staining in intact cytoplasm, with possible nuclear signal, as anatomically plausible for DUSP10 (UniProt Q9Y6W6; HPA tissue IHC). Check cell identity: high staining is reported in glandular cells of several tissues and neuronal cells of cerebral cortex, whereas low staining is reported in fibroblasts and adipocytes (HPA tissue IHC). A discordant cell pattern needs closer review, especially if signal concentrates at section edges, tissue folds, or necrotic areas (HPA tissue IHC; standard IHC practice). Compare no-primary and peroxidase-block controls to identify detection background or endogenous enzyme activity (standard IHC practice). Interpret a reproducible cellular pattern with preserved morphology more confidently than isolated brown deposits (standard IHC practice).
Boster reagents
Best DUSP10 / Dual specificity protein phosphatase 10 IHC Antibodies
One anti-DUSP10 antibody has IHC image data from paraffin-embedded human meningioma (A05412 image caption). Human, mouse and rat reactivity is listed (A05412 catalog: reactivity).
Real IHC data
Anti-MKP-5 DUSP10 Antibody
Cat # A05412
A05412 is listed for IHC and for human, mouse and rat reactivity (A05412 catalog: applications, reactivity). Its IHC image documents paraffin-embedded human meningioma with 1:200 primary dilution and Tris-EDTA retrieval at pH 9.0 (A05412 image caption).
Which to pick: For tissue IHC, choose A05412 for paraffin sections; its image does not report the fixative (A05412 image caption). No listed SKU has an IF/ICC application or IF image (A05412 catalog: applications, IF images). For mouse or rat IHC, A05412 lists both species and IHC, although its image shows human tissue only; the host is rabbit and clonality is unreported (A05412 catalog: reactivity, applications, host, clone; A05412 image caption).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q9Y6W6 (DUS10_HUMAN, Dual specificity protein phosphatase 10).
- Human Protein Atlas. DUSP10 tissue IHC expression (reliability: Approved).
- Human Protein Atlas. DUSP10 subcellular location (ICC-IF): Localized to the nucleoplasm and cytosol..
- Human Protein Atlas. DUSP10 antibody validation summary (1 antibodies).
- Colorectal Cancer-Associated Genes Are Associated with Tooth Agenesis and May Have a Role in Tooth Development. Scientific reports 2018 — PMC5813178.
- Transcriptomic Properties of HER2+ Ductal Carcinoma In Situ of the Breast Associate with Absence of Immune Cells. Biology 2021 — PMC8389698.
- Tumor Promoting Function of DUSP10 in Non-Small Cell Lung Cancer Is Associated With Tumor-Promoting Cytokines. Immune network 2023 — PMC10475826.
- DUSP10 Is a Regulator of YAP1 Activity Promoting Cell Proliferation and Colorectal Cancer Progression. Cancers 2019 — PMC6896144.
- PubMed PMID:10391943 — UniProt-cited evidence.
- PubMed PMID:10597297 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for DUSP10 (Dual specificity protein phosphatase 10). Expected localisation Cytoplasmic in tissue (HPA tissue IHC); nuclear possible (UniProt). Antigen retrieval: Tris-EDTA pH 9.0 HIER, heat-mediated (datasheet A05412). Validated IHC antibody: A05412 at 1:50 recommended; image 1:200 (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative None in HPA (detected in all 45 tissues); use no-primary + isotype controls. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded human meningioma tissue; fixative not specified (datasheet A05412); 2. Fixation: Image fixative and duration unreported (datasheet A05412); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Tris-EDTA pH 9.0 (datasheet A05412); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit anti-DUSP10, 1:50 recommended; image 1:200 (datasheet A05412); 9. Primary incubation: Overnight at 4 °C (standard). Watch for: Fixation: Keep fixation consistent across sections (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A05412); Caveat: Staining and RNA show medium consistency (HPA tissue IHC); Isoform / epitope: 2 isoforms; epitope coverage needs checking (UniProt); Regulation: Staining regulation not specified (UniProt). Evidence: UniProt Q9Y6W6, HPA tissue IHC · Approved, HPA ICC-IF · nucleoplasm and cytosol., Datasheet A05412, PMC8389698, PMC10475826. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/DUSP10/immunohistochemistry-ihc-guide.