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- Table of Contents
Designing a Western blot for EHD1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EHD1 Western Blot GuideFacts about EH domain-containing protein 1.
Recruited into endosomal membranes upon nerve growth factor stimulation, indirectly modulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (By similarity).
| Human | |
|---|---|
| Gene Name: | EHD1 |
| Uniprot: | Q9H4M9 |
| Entrez: | 10938 |

| Belongs to: |
|---|
| TRAFAC class dynamin-like GTPase superfamily |

EH-domain containing 1
Mass (kDA):
60.627 kDA

| Human | |
|---|---|
| Location: | 11q13.1 |
| Sequence: | 11; NC_000011.10 (64851642..64879713, complement) |
Highly expressed in testis.
Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, cilium membrane; Peripheral membrane protein; Cytoplasmic side. Preferentially associates with tubular recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:19864458, PubMed:23596323). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity). Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes





PMID: 9253601 by Guru S.C., et al. A transcript map for the 2.8-Mb region containing the multiple endocrine neoplasia type 1 locus.
PMID: 10395801 by Mintz L., et al. EHD1 -- an EH-domain-containing protein with a specific expression pattern.