Separin Antibodies

AND ESPL1 ELISA kits, Separin Proteins

Many biological assays use antibodies to detect Separin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Separin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Separin antibodies...

We validate the specificity of these antibodies to Separin by testing them on tissues known to express ESPL1 positively and negatively. Browse below to find the ESPL1 antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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ESPL1 Infographic by Boster Bio

All ESPL1 Products

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Anti-Separase Antibody

13 Q&As

Western Blot analysis of A431 cells using Separase Polyclonal Antibody diluted at 1:1000

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE Antibody

Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody

Human, Mouse

ELISA, IF, IHC, WB

$399
Cat # A04364
100μl

SEPARASE Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells

Human, Mouse

ELISA

$480
Cat # EKC1516
1 kit, containing one 96-well plate and all necessary reagents

SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30

Human, Mouse

ELISA

$540
Cat # EKC2000
1 kit, containing two 96-well plates and all necessary reagents

Anti-Separase (A795) ESPL1 Antibody

Western blot (WB) analysis of Separase (A795) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate

Immunohistochemistry (IHC) analyzes of Separase (A795) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IF, IHC, WB

$405
Cat # A04364-1
100ul

Anti-Phospho-Separase (S801) ESPL1 Antibody

Western Blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody

Human, Mouse

ELISA, IF, IHC, WB

$380
Cat # A04364S801
100ul

ESPL1 Overview

Facts about Separin.

ESPL1 3D structure. Source: alphafold

Separin (ESPL1)

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

Gene Information

Human
Gene Name:	ESPL1
Uniprot:	Q14674
Entrez:	9700

Family

Belongs to:

No superfamily

Alternative Names

Caspase-like protein ESPL1; EC 3.4.22.49; ESP1extra spindle poles like 1; extra spindle pole bodies homolog 1 (S. cerevisiae); extra spindle poles like 1 (S. cerevisiae); Extra spindle poles-like 1 protein; FLJ46492; KIAA0165separin; Separase