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- Table of Contents
F2RL1 · Western blot design guide
F2RL1 Western Blot Planning Guide
Plan a F2RL1 Western blot around the catalog-observed 44.1 kDa band, image-backed PB9700 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
F2RL1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 44.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Stomach | |
| Negative control | Oral mucosa |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 44.1 kDa |
| ⓘ | Localization | Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Sample controls for F2RL1 Western blot
🧪Use Stomach as the first positive-control candidate and Oral mucosa as the HPA Not detected negative candidate.
Positive control: Stomach (High)
Negative control: Oral mucosa (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for F2RL1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Stomach | Reported tissue cells | High | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 1
What Is the Expected F2RL1 Western Blot Band Size?
Use the product-observed 44.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 44.1 kDa | Matches the authoritative product WB observation. |
| 44.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected F2RL1 appearancePlan around 44.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 44.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 44.1 kDa from UniProt P55085; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9700. |
| Situation | Likely cause | Next action |
|---|---|---|
| 44.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing F2RL1 band
PB9700 · F2RL1Observed 44.1 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated F2RL1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Duodenum |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9700 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced F2RL1 Western Blot Tips
Deeper troubleshooting and optimisation questions for F2RL1, answered from its protein features.
Which band should guide the blot?
Use 44.1 kDa, the observation attached to the authoritative PB9700 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 44.1 kDa expectation.
Which positive control should I start with?
Start with Stomach, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Oral mucosa as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for F2RL1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9700 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC7156842, PMC12459351, PMC11821865.
Boster reagents
F2RL1 Western Blot Reagents
Human-reactive F2RL1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-PAR2/F2RL1 Antibody Picoband®
Cat # PB9700
Reactivity: Human
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P55085
- Human Protein Atlas — F2RL1
- PB9700 product record
- PMC7156842 — Protease-activated receptor-2 accelerates intestinal tumor formation through activation of nuclear factor-κB signaling and tumor angiogenesis in Apc(Min/+) mice.
- PMC12459351 — Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via protease activated receptor 2.
- PMC11821865 — Bioinformatics and experimental insights into F2RL1 as a key biomarker in cervical cancer diagnosis and prognosis.