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- Table of Contents
1 Citations 7 Q&As
6 Citations 8 Q&As
2 Citations 4 Q&As
4 Citations
Facts about Tumor necrosis factor receptor superfamily member 6.
Receptor for TNFSF6/FASLG.
The adapter molecule FADD recruits caspase-8 to the activated receptor.The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of older T-cells, or both.
Human | |
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Gene Name: | FAS |
Uniprot: | P25445 |
Entrez: | 355 |
Belongs to: |
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No superfamily |
ALPS1A; APO-1 cell surface antigen; Apo-1; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; APT1; CD95 antigen; CD95; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
Mass (kDA):
37.732 kDA
Human | |
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Location: | 10q23.31 |
Sequence: | 10; NC_000010.11 (88968429..89017059) |
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
The Boster Bio: Best Uses of the FAS Marker is a great IHC protocol book, which comes with a visual workflow and recommended reagents. This book was designed by Steven Boster, a biomedical engineer, who is known as the "he who transforms science into the lavatory". In the mid-90s, Boster was the largest antibody catalog company in China. PicoKine(tm), his own proprietary ELISA platform, was also invented by Boster. Its proprietary technology provides high-sensitivity ELISA kits, as well as detailed information about the background and use of the FAS marker.
The IHC agents used for this analysis are primary antibodies as well as secondary antibodies. The primary antibodies recognize epitopes within samples that the secondary antibody binds to. This process is known as indirect immunohistochemistry. The secondary antibody is made by immunizing a different host animal with the primary antibody. The immunization of the secondary antibody may be done in two ways.
IHC can detect both the denatured and native forms of an antigen. A positive control is necessary to ensure that the results are accurate. Antibodies should be stored according to the manufacturer's instructions. They should be kept away from light and contamination. Background staining can obscure the most important features of a tissues. Background staining can also be caused by improper binding of antibodies, or by mistakes when preparing slides for analysis.
There are many fixation methods available for intracellular staining. Depending on the type of antigens being stained, proteases may be used to break bridges between methylene. Bromelin and pepsin are two common mitogens that are used to remove antigens from the intracellular substance. The time of reaction for worn tissues may be extended. Moreover, fixed tissues must be stored at temperatures that are low.
BosterBio IHC Guide provides a step-by-step IHC protocol, as well as an easily illustrated IHC workflow and suggested materials. In addition, Boster also offers comprehensive technical information and blogs that provide information on various aspects of IHC. It is a valuable tool for advancing your career and learning the art of immunohistochemistry.
To get the most precise results, try different capture and detection antibodies. The antibodies should recognize distinct epitopes that are not overlapping. Otherwise, the results may be saturated. Additionally using the same antibody for both detection and capture may limit the dynamic range and sensitivity. Boster Bio also offers detailed troubleshooting guidance that will aid you in optimizing your experiments and get better results.
Indirect immunofluorescence staining involves staining target cells with a fluorescent secondary antibody. This method involves incubating cells with an antibody that has been linked to an afluorophore. This technique eliminates the possibility of non-specific binding due to large antibodies-fluorophore complexes. It also eliminates the use of secondary antibodies that fail to get into the cell.
Selecting a secondary antigen is an additional step. The majority of primary antibodies detect an antigen that is specific to. However, a secondary antibody should first be able to be pre-adsorbed against immunoglobulin of that antigen. The secondary antibody should be diluted and mounted using serum from the same species. If it is possible the secondary antibody must be created against a different species. Before the primary antibody is incubated it is essential to perform a biotin block.
The Peptide Coupler is among the IHC reagents we recommend. The peptide couplers can be employed in organic synthesis particularly in the pharmaceutical industry. The peptides are made from an amino acid sequence that are bonded together to form polypeptides. It is a complicated process that requires several steps. The BosterBio IHC procedure is well documented.
The story of Steve Boster begins in 1993 when he came up with his first product. He was nicknamed "the man who converts science in the lavatory" and towards the end of 90s, he'd developed hundreds of primary antibodies. PicoKine(tm) was his own ELISA platform. PicoKine(tm) is a unique ELISA platform that offers high-sensitivity ELISA kits. Steven Boster's company now has a complete line of ELISA kit and other products after years of development.
When using the FAS Marker in immunohistochemistry, there are several ways to optimize the results. In addition to a comprehensive IHC workflow, BosterBio also contains helpful information on preparation of samples, IHC sample design, and troubleshooting. This comprehensive guide covers all these important subjects. The Boster Bio provides practical advice on IHC, and the Boster BIO FAS Marker. It is an essential source for beginners as well as experts in immunostaining.
Boster provides high-affinity primary antibodies that have been tested for use in immunohistochemistry and Western Blotting. The Boster antibodies have been validated for use in these applications and are cited widely in journals of science. They also have been used for many years in other fields, such as Immunohistochemistry. Boster is a reputable brand in the research community.
PMID: 1713127 by Itoh N., et al. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis.
PMID: 1375228 by Oehm A., et al. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen.
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