Anti-Fas Antibody

Boster Bio Anti-Fas Antibody catalog # PA1119. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 21 publication(s).

Product Info Summary

SKU: PA1119
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Fas Antibody

See all Fas products

SKU/Catalog Number

PA1119

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fas Antibody catalog # PA1119. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fas Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1119)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1119 is reactive to Fas in Mouse, Rat

Applications

PA1119 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat , By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat , -
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Fas (Source: Uniprot.Org, NCBI)

Uniprot ID

Q63199

Gene ID

246097

Gene Name

Fas

Full Name

Tumor necrosis factor receptor superfamily member 6

Weight

36.835kDa

Alternative Names

ALPS1A; APO-1 cell surface antigen; Apo-1; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; APT1; CD95 antigen; CD95; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6 Fas|Tnfrsf6|Fas cell surface death receptor|tumor necrosis factor receptor superfamily member 6|CD95 antigen|FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas antigen (ATP1)|Fas receptor|Tumor necrosis factor receptor superfamily, member 6|apo-1 antigen|apoptosis-mediating surface antigen FAS

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fas, check out the Fas Infographic

Fas infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fas: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1119 has been cited in 21 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lanfen Chen,Wei Chen,Mengbei Zhang et al.Comparison of therapeutic effects of melatonin by two different routes in focal cerebral ischemic rats.Journal of Neurorestoratology 2019,07(01):47-53.
Species: Rat
PA1119 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Yan Zhang,Linchao Zhang,JiaLu Bao et al.Perfluorooctanoic Acid Exposure in Early Pregnancy Induces Oxidative Stress in The Uterus and Liver in Mice,13 February 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-160447
Species: Mouse
PA1119 usage in article: APP:IHC, SAMPLE:UTREUS, DILUTION:NA

Osteoprotegerin Induces Apoptosis of Osteoclasts and Osteoclast Precursor Cells via the Fas/Fas Ligand Pathway

HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1

Construction and identification of Fas-targeting siRNA-expressing plasmid

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues

Yeung CK, Wang G, Yao Y, Liang J, Tenny Chung CY, Chuai M, Lee KK, Yang X. Cell Death Dis. 2017 Mar 23;8(3):e2697. doi: 10.1038/cddis.2017.91. BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse

Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, Chen L, Liu C, Chen D, Chen L. Osteoarthritis Cartilage. 2014 Aug;22(8):1197-205. Doi: 10.1016/J.Joca.2014.06.031. Epub 2014 Jul 4. Loss Of Vhl In Cartilage Accelerated The Progression Of Age-Associated A...

Li W, Xia D, Wang Y, Li Y, Xue Y, Wu X, Ye Z. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):794-8. Doi: 10.1007/S11596-011-0679-6. Epub 2011 Dec 16. Relationship Between Aberrant Methylation Of Fas Promoter And Biological Behavior Of Bladd...

Liu Wl, Liu Zw, Li Ts, Wang C, Zhao B. Chin Med J (Engl). 2013 Feb;126(3):494-9. Hydrogen Sulfide Donor Regulates Alveolar Epithelial Cell Apoptosis In Rats With Acute Lung Injury.

Ma H, Zhang X, Zhang X, Yang D, Meng L, Zhang Y, Zhou S. Arch Med Sci. 2013 Apr 20;9(2):354-60. Doi: 10.5114/Aoms.2012.31439. Epub 2012 Oct 30. The Effect Of Esculentoside A On Lupus Nephritis-Prone Bxsb Mice.

Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Pro...

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

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17 Customer Q&As for Anti-Fas Antibody

Question

Does anti-Fas antibody PA1119 work for IHC with right ovary?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

According to the expression profile of right ovary, FAS is highly expressed in right ovary. So, it is likely that anti-Fas antibody PA1119 will work for IHC with right ovary.

Boster Scientific Support

Answered: 2020-01-10

Question

We need to test anti-Fas antibody PA1119 on rat right ovary for research purposes, then I may be interested in using anti-Fas antibody PA1119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The products we sell, including anti-Fas antibody PA1119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-13

Question

I was wanting to use your anti-Fas antibody for IHC for rat right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat right ovary identification?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

You can see on the product datasheet, PA1119 anti-Fas antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-06

Question

I am interested in using your anti-Fas antibody for negative regulation of schwann cell migration studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Thank you for your inquiry. This PA1119 anti-Fas antibody is validated on mouse intestine tissue, rat spleen tissue, nih3t3 whole cell lysates, tissue lysate. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-09

Question

My question regarding product PA1119, anti-Fas antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1119 anti-Fas antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-20

Question

Here is the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PA1119. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-09

Question

Would anti-Fas antibody PA1119 work on horse IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

Our lab technicians have not validated anti-Fas antibody PA1119 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Fas antibody PA1119 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-02

Question

Is a blocking peptide available for product anti-Fas antibody (PA1119)?

C. Jha

Verified customer

Asked: 2019-08-22

Answer

We do provide the blocking peptide for product anti-Fas antibody (PA1119). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-22

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PA1119. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-24

Question

Would PA1119 anti-Fas antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

You can see on the product datasheet, PA1119 anti-Fas antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-24

Question

I see that the anti-Fas antibody PA1119 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-10

Question

We bought anti-Fas antibody for WB on peripheral blood lymphocyte last year. I am using rat, and We are going to use the antibody for IHC next. I was wanting to use examining peripheral blood lymphocyte as well as urinary bladder in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

I have checked the website and datasheets of our anti-Fas antibody and it appears that PA1119 has been tested on rat in both WB and IHC. Thus PA1119 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-03

Question

Do you have a BSA free version of anti-Fas antibody PA1119 available?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fas antibody PA1119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-20

Question

Our lab were satisfied with the WB result of your anti-Fas antibody. However we have seen positive staining in cervix carcinoma isoform 2: secreted. using this antibody. Is that expected? Could you tell me where is FAS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

From literature, cervix carcinoma does express FAS. Generally FAS expresses in isoform 1: cell membrane, isoform 2: secreted., isoform 3: secreted., isoform 4: secreted., isoform 5: secreted., isoform 6: secreted. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-07-21

Question

We are currently using anti-Fas antibody PA1119 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?

P. Taylor

Verified customer

Asked: 2017-01-17

Answer

The anti-Fas antibody (PA1119) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-01-17

Question

We have been able to see staining in rat cervix carcinoma. Any tips? Is anti-Fas antibody supposed to stain cervix carcinoma positively?

J. Bhatt

Verified customer

Asked: 2014-10-23

Answer

Based on literature cervix carcinoma does express FAS. Based on Uniprot.org, FAS is expressed in right ovary, peripheral blood lymphocyte, urinary bladder, cervix carcinoma, liver, among other tissues. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-10-23

Question

Is this PA1119 anti-Fas antibody reactive to the isotypes of FAS?

D. Anderson

Verified customer

Asked: 2014-10-23

Answer

The immunogen of PA1119 anti-Fas antibody is A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-10-23

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PA1119
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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