|Product Name||Anti-Fas Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.|
|Cite This Product||Anti-Fas Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1119)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL).|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat , By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat , -
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti-Fas antibody, PA1119, Western blotting
Lane 1: Recombinant Mouse FAS Protein 10ng
Lane 2: Recombinant Mouse FAS Protein 5ng
Lane 3: Recombinant Mouse FAS Protein 2.5ng
Anti-Fas antibody, PA1119, IHC(P)
IHC(P): Rat Spleen Tissue Lysate
Western blot analysis of FAS expression in rat liver extract (lane 1), rat spleen extract (lane 2), rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Western blot analysis of FAS expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse brain extract (lane 3) mouse kidney extract (lane 4), mouse thymus extract (lane 5), mouse lung extract (lane 6), HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tumor necrosis factor receptor superfamily member 6|
|Alternative Names||Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;Fas;Apt1, Tnfrsf6;|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Molecular Weight||36835 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). .|
|Research Areas||Adaptive Immunity, Apoptosis, Autoimmune, Cell Biology, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, Tnf Superfamily
*You can search these to find other products in these research areas.
|Background||FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,