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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
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The following reagents are used to generate the images below.
Anti-Fas antibody, PA1119, Western blottingLane 1: Recombinant Mouse FAS Protein 10ngLane 2: Recombinant Mouse FAS Protein 5ngLane 3: Recombinant Mouse FAS Protein 2.5ng
Western blot analysis of FAS expression in rat liver extract (lane 1), rat spleen extract (lane 2), rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Western blot analysis of FAS expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse brain extract (lane 3) mouse kidney extract (lane 4), mouse thymus extract (lane 5), mouse lung extract (lane 6), HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Anti-Fas antibody, PA1119, IHC(P)IHC(P): Rat Spleen Tissue Lysate
Figure 5. IHC analysis of FAS using anti-FAS antibody (PA1119). FAS was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FAS Antibody (PA1119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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