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- Table of Contents
G3BP1 · Western blot design guide
G3BP1 Western Blot Planning Guide
Plan a G3BP1 Western blot around the catalog-observed 52.2 kDa band, image-backed A02199-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
G3BP1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 52.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cervix | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 52.2 kDa |
| ⓘ | Localization | Cytoplasm, cytosol / Perikaryon |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Sample controls for G3BP1 Western blot
🧪Use Cervix as the first positive-control candidate and Heart muscle as the HPA Not detected negative candidate.
Positive control: Cervix (High)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for G3BP1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Esophagus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 1
What Is the Expected G3BP1 Western Blot Band Size?
Use the product-observed 52.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 52.2 kDa | Matches the authoritative product WB observation. |
| 52.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected G3BP1 appearancePlan around 52.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 52.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 52.2 kDa from UniProt Q13283; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02199-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 52.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing G3BP1 band
A02199-2 · G3BP1Observed 52.2 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated G3BP1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02199-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced G3BP1 Western Blot Tips
Deeper troubleshooting and optimisation questions for G3BP1, answered from its protein features.
Which band should guide the blot?
Use 52.2 kDa, the observation attached to the authoritative A02199-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 52.2 kDa expectation.
Which positive control should I start with?
Start with Cervix, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for G3BP1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02199-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC12421999, PMC13388048.
Boster reagents
G3BP1 Western Blot Reagents
Human-reactive G3BP1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-G3BP/G3BP1 Antibody Picoband®
Cat # A02199-2
Reactivity: Human
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13283
- Human Protein Atlas — G3BP1
- A02199-2 product record
- PMC12421999 — Convergent evolution of the G3BP1-binding motif in betacoronavirus nucleocapsid proteins.
- PMC13388048 — G3BP1 methylation by PRMT5 facilitates its recruitment of TBK1 for IRF3 activation during host response to foreign ribonucleic acids.