GAR1 · Western blot design guide

GAR1 Western Blot Planning Guide

Plan a GAR1 Western blot around the catalog-observed 22.3 kDa band, image-backed A07049-1 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for GAR1 (GAR1): expected band 22.3 kDa, antibody A07049-1, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

GAR1 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	22.3 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Breast
	Negative control ⓘ	Adipose tissue

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	22.3 kDa
ⓘ	Localization	Nucleus, nucleolus / Nucleus, Cajal body
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Sample controls for GAR1 Western blot

🧪Use Breast as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.

Positive control: Breast (High)

Negative control: Adipose tissue (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for GAR1

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Breast	Reported tissue cells	High	Protein (HPA)	HPA →
Adrenal gland	Reported tissue cells	High	Protein (HPA)	HPA →
Appendix	Reported tissue cells	High	Protein (HPA)	HPA →
Bone marrow	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected GAR1 Western Blot Band Size?

Use the product-observed 22.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
22.3 kDa	Matches the authoritative product WB observation.
22.3 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected GAR1 appearancePlan around 22.3 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	22.3 kDa; use this as the primary experimental expectation.
Calculated mass	22.3 kDa from UniProt Q9NY12; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A07049-1.

Why is my band missing or off?
Situation	Likely cause	Next action
22.3 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q9NY12 Human Protein Atlas product datasheet (A07049-1)

Experiment evidence

Western blot images showing GAR1 band

A07049-1 · GAR1 — observed 22.3 kDa WB validation

A07049-1 · GAR1Observed 22.3 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated GAR1 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adrenal gland
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, short transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A07049-1 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced GAR1 Western Blot Tips

Deeper troubleshooting and optimisation questions for GAR1, answered from its protein features.

Which band should guide the blot?

Use 22.3 kDa, the observation attached to the authoritative A07049-1 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 22.3 kDa expectation.

Which positive control should I start with?

Start with Breast, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Adipose tissue as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for GAR1 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A07049-1 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC10508036, PMC10840380, PMC8170401.

Boster reagents

GAR1 Western Blot Reagents

Human-reactive GAR1 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for GAR1 using A07049-1; observed band 22.3 kDa

Anti-NOLA1/GAR1 Antibody Picoband®

Cat # A07049-1

Reactivity: Human

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.