GATA6 · Western blot design guide

GATA6 Western Blot Planning Guide

Plan a GATA6 Western blot around the catalog-observed 60 kDa band, image-backed A00778-3 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for GATA6 (GATA6): expected band 60 kDa, antibody A00778-3, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

GATA6 Western Blot Experimental Design Guide

Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	60 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Gallbladder
	Negative control ⓘ	Cerebral cortex

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	60 kDa
ⓘ	Localization	Nucleus
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Sample controls for GATA6 Western blot

🧪Use Gallbladder as the first positive-control candidate and Cerebral cortex as the HPA Not detected negative candidate.

Positive control: Gallbladder (High)

Negative control: Cerebral cortex (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for GATA6

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Gallbladder	Reported tissue cells	High	Protein (HPA)	HPA →
Pancreas	Reported tissue cells	High	Protein (HPA)	HPA →
Colon	Reported tissue cells	High	Protein (HPA)	HPA →
Duodenum	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Cerebral cortex	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Heart muscle	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected GATA6 Western Blot Band Size?

Use the product-observed 60 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
60 kDa	Matches the authoritative product WB observation.
60 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected GATA6 appearancePlan around 60 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	60 kDa; use this as the primary experimental expectation.
Calculated mass	60 kDa from UniProt Q92908; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A00778-3.

Why is my band missing or off?
Situation	Likely cause	Next action
60 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q92908 Human Protein Atlas product datasheet (A00778-3)

Experiment evidence

Western blot images showing GATA6 band

A00778-3 · GATA6 — observed 60 kDa WB validation

A00778-3 · GATA6Observed 60 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated GATA6 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Colon
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A00778-3 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced GATA6 Western Blot Tips

Deeper troubleshooting and optimisation questions for GATA6, answered from its protein features.

Which band should guide the blot?

Use 60 kDa, the observation attached to the authoritative A00778-3 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 60 kDa expectation.

Which positive control should I start with?

Start with Gallbladder, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Cerebral cortex as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for GATA6 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A00778-3 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC5354868, PMC9118154.

Boster reagents

GATA6 Western Blot Reagents

Human-reactive GATA6 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for GATA6 using A00778-3; observed band 60 kDa

Anti-GATA6 Antibody Picoband®

Cat # A00778-3

Reactivity: Human

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.