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- Table of Contents
GOT2 · Western blot design guide
GOT2 Western Blot Planning Guide
Plan a GOT2 Western blot around the catalog-observed 47.5 kDa band, image-backed A05998-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
GOT2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 47.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cerebellum | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 47.5 kDa |
| ⓘ | Localization | Mitochondrion matrix / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated GOT2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebellum |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A05998-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected GOT2 Western Blot Band Size?
Use the product-observed 47.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 47.5 kDa | Matches the authoritative product WB observation. |
| 47.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected GOT2 appearancePlan around 47.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 47.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 47.5 kDa from UniProt P00505; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A05998-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 47.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing GOT2 band
A05998-1 · GOT2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for GOT2 Western blot
🧪Use Cerebellum as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Cerebellum (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for GOT2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Hippocampus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
| Epididymis | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced GOT2 Western Blot Tips
Deeper troubleshooting and optimisation questions for GOT2, answered from its protein features.
Which band should guide the blot?
Use 47.5 kDa, the observation attached to the authoritative A05998-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 47.5 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for GOT2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A05998-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
GOT2 Western Blot Reagents
Human/Mouse/Rat-reactive GOT2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-FABP-1/GOT2 Antibody Picoband®
Cat # A05998-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P00505
- Human Protein Atlas — GOT2
- A05998-1 product record
- PMC8806680 — Circular RNA circ_0003028 contributes to tumorigenesis by regulating GOT2 via miR-1298-5p in non-small cell lung cancer (Bioengineered, 2021)
- PMC12255758 — Glucose-induced STUB1-GOT2 axis promotes aspartate synthesis and mitochondrial dysfunction in bladder cancer (Cell death & disease, 2025)
- PMC9328765 — Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context (eLife, 2022)