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- Table of Contents
Facts about Histone H2B type 2-F.
Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Human | |
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Gene Name: | HIST2H2BF |
Uniprot: | Q5QNW6 |
Entrez: |
Belongs to: |
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histone H2B family |
FLJ35099; FLJ56780; FLJ56787; HIST1H2BK; histone 2, H2bf; histone cluster 2, H2bf; histone H2B type 2-F; Histone H2B; MGC131639
Mass (kDA):
13.92 kDA
Human | |
---|---|
Location: | |
Sequence: | ; |
Nucleus. Chromosome.
You have reached the right place if your goal is to find a reliable primary antibody for HIST2H2BF detection. Boster's high-affinity antibodies are well-respected and have been extensively referenced in the research community for the past 25 years. You can trust their products because they've been validated in Western Blotting, Immunohistochemistry, and ELISA.
Recent studies suggest that HIST2H2BF may play a critical role in tumor initiation or development. The HIST2H2BF gene has high expression in tumor stem cells. Researchers separated CRC cell lines using sphere-forming assays. They also distinguished CSCs from non-tumor-stem cells. High-expressing cell lines had higher HIST2H2BF Expression, and knockdown cells were established from LOVO Cells.
The HIST2H2BF gene has been associated with colorectal cancer (CRC). It is not clear how these cancer stem cells can maintain these properties. Researchers have discovered that HIST2H2BF expression accelerates the progression of CRC by maintaining stem cell features. Further research is needed on this genetic marker. However, there are promising areas that can be further investigated.
The HIST2H2BF genes was found to be overexpressed in CRC as well as adjacent normal tissues. This marker can be detected using Western blotting or real-timePCR. In addition, researchers used lentivirus to promote tumor formation in mice. They also measured tumor weight and frequency of CSCs two months after injection of the HIST2H2BF gene. They also examined tumor weights in LOVO cells and SW480 cells.
Researchers have also found that HIST2H2BF can be expressed in CRC cell lines by either overexpression or knockdown. HIST2H2BF can be inhibited to increase CRC cell apoptosis. Downregulation of the gene results in decreased proliferation and invasiveness. These findings highlight potential therapeutic targets for CRC. These data can be accessed online from repositories.
Boster Bio has developed and produced a line of high-affinity primary antibodies that are well cited and trusted in the research community. These antibodies are tested for a broad range of applications, including Western Blotting, Immunohistochemistry, and ELISA. The HIST2H2BF antibody can be used to detect the presence of the appropriate protein in human samples.
Primary antibodies are a subset among immunoglobulins and can only bind to one antigen. The strength of this bond is known as affinity, and specificity is the amount of binding to unintended antigens. Good primary antibodies are able to detect, purify, measure, and quantify the antigen of your interest. Here's how to recognize them.
Researchers can label specimens using the Boster Bio HIST2H2BF's high-affinity primary antibodies system. Scientists can ask more questions by using both primary antibodies as well as secondary antibodies. The result is more robust data and contextual information. Boster Bio has created the HIST2H2BF marker, which is high-affinity prima antibodies.
Monoclonal antibody are used as primary antibodies in research. Their characteristics include high specificity for an epitope of an antigen, low non-specific cross-reactivity, and minimal lot-to-lot variation. Monoclonal antibodies were traditionally developed in mice for various uses, but new techniques have made them possible to be raised in other species.
The HIST2H2BF gene encodes protein and is located on chromosome 1. This gene is found in humans, Rhesus monkeys, cows, mice, and rats. It also has orthologs that can be found in 1 organism (human, mouse, rat and chimpanzee). This information is only for reference. Although HIST2H2BF is not expressed in all tissues of the body, its upregulation is associated both with liver metastasis as well as increased malignancy.
PMID: 12757711 by Cheung W.L., et al. Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase.
PMID: 16307923 by Zhu B., et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation.